Cell Biology Antibodies 4
Anti-CA7 Antibody (CAB14355)
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- SKU:
- CAB14355
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CA7 Antibody |
| Antibody SKU: | CAB14355 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human CA7 (NP_005173.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Mouse brain, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-264 of human CA7 (NP_005173.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTGH HGWG YGQD DGPS HWHK LYPI AQGD RQSP INII SSQA VYSP SLQP LELS YEAC MSLS ITNN GHSV QVDF NDSD DRTV VTGG PLEG PYRL KQFH FHWG KKHD VGSE HTVD GKSF PSEL HLVH WNAK KYST FGEA ASAP DGLA VVGV FLET GDEH PSMN RLTD ALYM VRFK GTKA QFSC FNPK CLLP ASRH YWTY PGSL TTPP LSES VTWI VLRE PICI SERQ MGKF RSLL FTSE DDER IHMV NNFR PPQP LKGR VVKA SFRA |
| Gene ID: | 766 |
| Uniprot: | P43166 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 23kDa/29kDa |
| Observed MW: | 30kDa |
| Synonyms: | CA7, CAVII |
| Background: | Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | CA7: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase Chromosomal Location of Human Ortholog: 16q22.1 Cellular Component: cytosol Molecular Function:carbonate dehydratase activity; zinc ion binding Biological Process: bicarbonate transport; positive regulation of synaptic transmission, GABAergic; positive regulation of cellular pH reduction; one-carbon compound metabolic process |
| NCBI Summary: | Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P43166 |
| NCBI GenInfo Identifier: | 1168744 |
| NCBI Gene ID: | 766 |
| NCBI Accession: | P43166.1 |
| UniProt Secondary Accession: | P43166,Q541F0, Q86YU0, |
| UniProt Related Accession: | P43166 |
| Molecular Weight: | 23,452 Da |
| NCBI Full Name: | Carbonic anhydrase 7 |
| NCBI Synonym Full Names: | carbonic anhydrase VII |
| NCBI Official Symbol: | CA7 |
| NCBI Official Synonym Symbols: | CAVII |
| NCBI Protein Information: | carbonic anhydrase 7; CA-VII; carbonate dehydratase VII; carbonic dehydratase VII |
| UniProt Protein Name: | Carbonic anhydrase 7 |
| UniProt Synonym Protein Names: | Carbonate dehydratase VII; Carbonic anhydrase VII; CA-VII |
| Protein Family: | Carbonic anhydrase |
| UniProt Gene Name: | CA7 |
| UniProt Entry Name: | CAH7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CA7 antibody (CAB14355) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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