Cell Biology Antibodies 5

Anti-CA5A Antibody (CAB14709)

(No reviews yet) Write a Review
SKU:
CAB14709
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-CA5A Antibody
Antibody SKU:CAB14709
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 39-305 of human CA5A (NP_001730.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:K-562, LO2, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 39-305 of human CA5A (NP_001730.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CAWQ TSNN TLHP LWTV PVSV PGGT RQSP INIQ WRDS VYDP QLKP LRVS YEAA SCLY IWNT GYLF QVEF DDAT EASG ISGG PLEN HYRL KQFH FHWG AVNE GGSE HTVD GHAY PAEL HLVH WNSV KYQN YKEA VVGE NGLA VIGV FLKL GAHH QTLQ RLVD ILPE IKHK DARA AMRP FDPS TLLP TCWD YWTY AGSL TTPP LTES VTWI IQKE PVEV APSQ LSAF RTLL FSAL GEEE KMMV NNYR PLQP LMNR KVWA SFQA TNEG TRS
Gene ID:763
Uniprot:P35218
Cellular Location:Mitochondrion
Calculated MW:34kDa
Observed MW:35kDa
Synonyms:CA5A, CA5, CA5AD, CAV, CAVA, GS1-21A4.1
Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2.
UniProt Protein Function:Car5a: Reversible hydration of carbon dioxide. Low activity. Belongs to the alpha-carbonic anhydrase family.
UniProt Protein Details:

Protein type:EC 4.2.1.1; Lyase; Mitochondrial; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function:carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process

Disease: Carbonic Anhydrase Va Deficiency, Hyperammonemia Due To

NCBI Summary:Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. CA VA is localized in the mitochondria and expressed primarily in the liver. It may play an important role in ureagenesis and gluconeogenesis. CA5A gene maps to chromosome 16q24.3 and an unprocessed pseudogene has been assigned to 16p12-p11.2. [provided by RefSeq, Jul 2008]
UniProt Code:P35218
NCBI GenInfo Identifier:4502521
NCBI Gene ID:763
NCBI Accession:
UniProt Related Accession:P35218
Molecular Weight:
NCBI Full Name:carbonic anhydrase 5A, mitochondrial isoform 1
NCBI Synonym Full Names:carbonic anhydrase 5A
NCBI Official Symbol:CA5A  
NCBI Official Synonym Symbols:CA5; CAV; CAVA; CA5AD; GS1-21A4.1  
NCBI Protein Information:carbonic anhydrase 5A, mitochondrial
UniProt Protein Name:Carbonic anhydrase 5A, mitochondrial
UniProt Synonym Protein Names:Carbonate dehydratase VA; Carbonic anhydrase VA
Protein Family:Carbonic anhydrase
UniProt Gene Name:CA5A  
UniProt Entry Name:CAH5A_HUMAN
Anti-CA5A Antibody (CAB14709)Western blot analysis of extracts of various cell lines, using CA5A antibody (CAB14709) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
View AllClose

0 Reviews

View AllClose

Related Products

Human CA5A/CA-VA Recombinant Protein (RPES2596)

Human CA5A/CA-VA Recombinant Protein (RPES2596)

system_update_altDatasheetHuman CA5A/CA-VA Recombinant Protein Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member o