Cell Biology Antibodies 1
Anti-CA1 Antibody (CAB1031)
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- SKU:
- CAB1031
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-CA1 Antibody |
| Antibody SKU: | CAB1031 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human CA1 (NP_001122303.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:10 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Mouse liver, Mouse heart, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-261 of human CA1 (NP_001122303.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MASP DWGY DDKN GPEQ WSKL YPIA NGNN QSPV DIKT SETK HDTS LKPI SVSY NPAT AKEI INVG HSFH VNFE DNDN RSVL KGGP FSDS YRLF QFHF HWGS TNEH GSEH TVDG VKYS AELH VAHW NSAK YSSL AEAA SKAD GLAV IGVL MKVG EANP KLQK VLDA LQAI KTKG KRAP FTNF DPST LLPS SLDF WTYP GSLT HPPL YESV TWII CKES ISVS SEQL AQFR SLLS NVEG DNAV PMQH NNRP TQPL KGRT VRAS F |
| Gene ID: | 759 |
| Uniprot: | P00915 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 28kDa |
| Observed MW: | 30kDa |
| Synonyms: | CA1, CA-I, CAB, Car1, HEL-S-11 |
| Background: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing and the use of alternative promoters results in multiple transcript variants. |
| UniProt Protein Function: | CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family. |
| UniProt Protein Details: | Protein type:EC 4.2.1.1; Energy Metabolism - nitrogen; Lyase Chromosomal Location of Human Ortholog: 8q21.2 Cellular Component: cytosol Molecular Function:carbonate dehydratase activity; zinc ion binding Biological Process: bicarbonate transport; one-carbon compound metabolic process |
| NCBI Summary: | Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014] |
| UniProt Code: | P00915 |
| NCBI GenInfo Identifier: | 115449 |
| NCBI Gene ID: | 759 |
| NCBI Accession: | P00915.2 |
| UniProt Related Accession: | P00915 |
| Molecular Weight: | 261 |
| NCBI Full Name: | Carbonic anhydrase 1 |
| NCBI Synonym Full Names: | carbonic anhydrase I |
| NCBI Official Symbol: | CA1 |
| NCBI Official Synonym Symbols: | CAB; CA-I; Car1; HEL-S-11 |
| NCBI Protein Information: | carbonic anhydrase 1; carbonic anhydrase B; carbonate dehydratase I; epididymis secretory protein Li 11 |
| UniProt Protein Name: | Carbonic anhydrase 1 |
| UniProt Synonym Protein Names: | Carbonate dehydratase I; Carbonic anhydrase B; CAB; Carbonic anhydrase I; CA-I |
| Protein Family: | Cation-transporting ATPase |
| UniProt Gene Name: | CA1 |
| UniProt Entry Name: | CAH1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CA1 antibody (CAB1031) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of A-549 cells using CA1 antibody (CAB1031). Blue: DAPI for nuclear staining. |
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