Immunology Antibodies 3
Anti-C8B Antibody (CAB8324)
- SKU:
- CAB8324
- Product Type:
- Antibody
- Applications:
- WB
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-C8B Antibody |
| Antibody SKU: | CAB8324 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-350 of human C8B (NP_000057.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Jurkat, HeLa, THP-1, Raji, Mouse liver, Rat liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 55-350 of human C8B (NP_000057.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SVDV TLMP IDCE LSSW SSWT TCDP CQKK RYRY AYLL QPSQ FHGE PCNF SDKE VEDC VTNR PCRS QVRC EGFV CAQT GRCV NRRL LCNG DNDC GDQS DEAN CRRI YKKC QHEM DQYW GIGS LASG INLF TNSF EGPV LDHR YYAG GCSP HYIL NTRF RKPY NVES YTPQ TQGK YEFI LKEY ESYS DFER NVTE KMAS KSGF SFGF KIPG IFEL GISS QSDR GKHY IRRT KRFS HTKS VFLH ARSD LEVA HYKL KPRS LMLH YEFL QRVK RLPL EYSY GEYR DLFR DFGT HYIT EAVL |
| Gene ID: | 732 |
| Uniprot: | P07358 |
| Cellular Location: | Secreted |
| Calculated MW: | 67kDa |
| Observed MW: | 67kDa |
| Synonyms: | C8B, C82 |
| Background: | This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | C8B: Constituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. Defects in C8B are a cause of complement component 8 deficiency type 2 (C8D2). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. Belongs to the complement C6/C7/C8/C9 family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 1p32.2 Cellular Component: membrane attack complex; extracellular space; membrane; extracellular region; vesicle Molecular Function:protein complex binding Biological Process: cytolysis; complement activation, alternative pathway; regulation of complement activation; innate immune response; immune response; complement activation, classical pathway; complement activation Disease: Complement Component 8 Deficiency, Type Ii |
| NCBI Summary: | This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013] |
| UniProt Code: | P07358 |
| NCBI GenInfo Identifier: | 514239918 |
| NCBI Gene ID: | 732 |
| NCBI Accession: | NP_001265472.1 |
| UniProt Secondary Accession: | P07358,A1L4K7, |
| UniProt Related Accession: | P07358 |
| Molecular Weight: | 67,047 Da |
| NCBI Full Name: | complement component C8 beta chain isoform 2 |
| NCBI Synonym Full Names: | complement component 8, beta polypeptide |
| NCBI Official Symbol: | C8B |
| NCBI Official Synonym Symbols: | C82 |
| NCBI Protein Information: | complement component C8 beta chain; complement component 8 subunit beta |
| UniProt Protein Name: | Complement component C8 beta chain |
| UniProt Synonym Protein Names: | Complement component 8 subunit beta |
| Protein Family: | Complement C2 |
| UniProt Gene Name: | C8B |
| UniProt Entry Name: | CO8B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using C8B antibody (CAB8324) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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