KO Validated Antibodies 2
Anti-c-Myc Antibody [KO Validated] (CAB19032)
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![Anti-c-Myc Antibody [KO Validated] (CAB19032)](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/608x608/products/55796/60978/anti-c-myc-antibody-ko-validated-cab19032__93344__31381.1706533677.jpg?c=1 "Anti-c-Myc Antibody [KO Validated] (CAB19032)")
- SKU:
- CAB19032
![Anti-c-Myc Antibody [KO Validated] (CAB19032)](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/55796/60978/anti-c-myc-antibody-ko-validated-cab19032__93344__31381.1706533677.jpg?c=1?imbypass=on "Anti-c-Myc Antibody [KO Validated] (CAB19032)")
Description
| Antibody Name: | Anti-c-Myc Antibody [KO Validated] |
| Antibody SKU: | CAB19032 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthesized peptide derived from human c-Myc |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HCT116, Jurkat, MCF7, Mouse ovary |
| Immunogen: | A synthesized peptide derived from human c-Myc |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 4609 |
| Uniprot: | P01106 |
| Cellular Location: | Nucleus, nucleolus, nucleoplasm |
| Calculated MW: | 57kDa |
| Observed MW: | 50-60kDa |
![Western blot - [KO Validated] c-Myc Rabbit mAb](https://abclonal.com/Uploads/Catalog/A19032/A19032_1.jpg) | Western blot analysis of extracts of various cell lines, using c-Myc antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
![Western blot - [KO Validated] c-Myc Rabbit mAb](https://abclonal.com/Uploads/Catalog/A19032/A19032_2.jpg) | Western blot analysis of extracts of Mouse ovary, using c-Myc antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min. |
![Western blot - [KO Validated] c-Myc Rabbit mAb](https://abclonal.com/Uploads/Catalog/A19032/A19032_3.jpg) | Western blot analysis of extracts from normal (control) and c-Myc knockout (KO) HCT116 cells, using c-Myc antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
![Immunofluorescence - [KO Validated] c-Myc Rabbit mAb](https://abclonal.com/Uploads/Catalog/A19032/A19032_4.jpg) | Immunofluorescence analysis of HeLa cells using c-Myc Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
![Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb](https://abclonal.com/Uploads/Catalog/A19032/A19032_5.jpg) | Chromatin immunoprecipitation analysis of extracts of K-562 cells, using c-Myc antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
Additional Information
Product Type: |
Antibody |
Reactivity: |
Human |
Reactivity: |
Mouse |
Host Species: |
Rabbit |
Isotype: |
IgG |
Research Area: |
Epigenetics and Nuclear Signaling |
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