KO Validated Antibodies 2

Anti-c-Myc Antibody [KO Validated] (CAB19032)

(No reviews yet) Write a Review
SKU:
CAB19032
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-c-Myc Antibody [KO Validated]
Antibody SKU:CAB19032
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human c-Myc
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HCT116, Jurkat, MCF7, Mouse ovary
Immunogen:A synthesized peptide derived from human c-Myc
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4609
Uniprot:P01106
Cellular Location:Nucleus, nucleolus, nucleoplasm
Calculated MW:57kDa
Observed MW:50-60kDa
Western blot - [KO Validated] c-Myc Rabbit mAb Western blot analysis of extracts of various cell lines, using c-Myc antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Western blot - [KO Validated] c-Myc Rabbit mAb Western blot analysis of extracts of Mouse ovary, using c-Myc antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 3min.
Western blot - [KO Validated] c-Myc Rabbit mAb Western blot analysis of extracts from normal (control) and c-Myc knockout (KO) HCT116 cells, using c-Myc antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunofluorescence - [KO Validated] c-Myc Rabbit mAb Immunofluorescence analysis of HeLa cells using c-Myc Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation - [KO Validated] c-Myc Rabbit mAb Chromatin immunoprecipitation analysis of extracts of K-562 cells, using c-Myc antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
View AllClose

0 Reviews

View AllClose

Related Products