Cell Death Antibodies 1
Anti-BUB1B Antibody (CAB1775)
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- SKU:
- CAB1775
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-BUB1B Antibody |
| Antibody SKU: | CAB1775 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BUB1B (NP_001202.4). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HeLa, Jurkat, HT-29, A-549, COS-7, MCF7, DU145 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BUB1B (NP_001202.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAV KKEG GALS EAMS LEGD EWEL SKEN VQPL RQGR IMST LQGA LAQE SACN NTLQ QQKR AFEY EIRF YTGN DPLD VWDR YISW TEQN YPQG GKES NMST LLER AVEA LQGE KRYY SDPR FLNL WLKL GRLC NEPL DMYS YLHN QGIG VSLA QFYI SWAE EYEA RENF RKAD AIFQ EGIQ QKAE PLER LQSQ HRQF QARV SRQT LLAL EKEE EEEV FESS VPQR STLA ELKS KGKK TARA PIIR VGGA LKAP SQNR GLQN |
| Gene ID: | 701 |
| Uniprot: | O60566 |
| Cellular Location: | Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center |
| Calculated MW: | 105kDa/119kDa/121kDa |
| Observed MW: | 55kDa/120kDa |
| Synonyms: | BUB1B, BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1 |
| Background: | This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. |
| UniProt Protein Function: | BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Other group; BUB family Chromosomal Location of Human Ortholog: 15q15 Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity Biological Process: cell proliferation; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; apoptosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait |
| NCBI Summary: | This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O60566 |
| NCBI GenInfo Identifier: | 143811368 |
| NCBI Gene ID: | 701 |
| NCBI Accession: | O60566.3 |
| UniProt Secondary Accession: | O60566,O60501, O60627, O60758, O75389, Q59HH6, Q8WV50 Q96KM4, B2R6U0, B4DL09, B4DLG3, |
| UniProt Related Accession: | O60566 |
| Molecular Weight: | 1050 |
| NCBI Full Name: | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta |
| NCBI Synonym Full Names: | BUB1 mitotic checkpoint serine/threonine kinase B |
| NCBI Official Symbol: | BUB1B |
| NCBI Official Synonym Symbols: | MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta |
| NCBI Protein Information: | mitotic checkpoint serine/threonine-protein kinase BUB1 beta; mitotic checkpoint kinase MAD3L; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 homolog beta |
| UniProt Protein Name: | Mitotic checkpoint serine/threonine-protein kinase BUB1 beta |
| UniProt Synonym Protein Names: | MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1 |
| Protein Family: | Mitotic checkpoint serine/threonine-protein kinase |
| UniProt Gene Name: | BUB1B |
| UniProt Entry Name: | BUB1B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BUB1B antibody (CAB1775) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded human breast cancer using BUB1B antibody (CAB1775) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using BUB1B antibody (CAB1775) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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