Cell Death Antibodies 1

Anti-BUB1B Antibody (CAB14525)

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SKU:
CAB14525
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-BUB1B Antibody
Antibody SKU:CAB14525
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human BUB1B (NP_001202.4).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T, U-87MG, HeLa, K-562, Mouse thymus, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 160-400 of human BUB1B (NP_001202.4).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EEYE AREN FRKA DAIF QEGI QQKA EPLE RLQS QHRQ FQAR VSRQ TLLA LEKE EEEE VFES SVPQ RSTL AELK SKGK KTAR APII RVGG ALKA PSQN RGLQ NPFP QQMQ NNSR ITVF DENA DEAS TAEL SKPT VQPW IAPP MPRA KENE LQAG PWNT GRSL EHRP RGNT ASLI AVPA VLPS FTPY VEET AQQP VMTP CKIE PSIN HILS TRKP GKEE GDPL QRVQ SHQQ ASEE KKEK MMYC K
Gene ID:701
Uniprot:O60566
Cellular Location:Chromosome, Cytoplasm, Nucleus, centromere, centrosome, cytoskeleton, kinetochore, microtubule organizing center
Calculated MW:105kDa/119kDa/121kDa
Observed MW:130kDa
Synonyms:BUB1B, BUB1beta, BUBR1, Bub1A, MAD3L, MVA1, SSK1, hBUBR1
Background:This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.
UniProt Protein Function:BUB1B: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression. Interacts with CENPE, CENPF, mitosin, PLK1 and BUB3. Part of a complex containing BUB3, CDC20 and BUB1B. Interacts with anaphase-promoting complex/cyclosome (APC/C). Interacts with CASC5. Induced during mitosis. Highly expressed in thymus followed by spleen. Preferentially expressed in tissues with a high mitotic index. Kinase activity stimulated by CENPE. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 2.7.11.1; Protein kinase, Other; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor; Other group; BUB family

Chromosomal Location of Human Ortholog: 15q15

Cellular Component: kinetochore; anaphase-promoting complex; perinuclear region of cytoplasm; cytoplasm; microtubule organizing center; outer kinetochore of condensed chromosome; spindle midzone; cytosol

Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: cell proliferation; mitosis; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; cell division; apoptosis; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; protein amino acid phosphorylation; mitotic metaphase/anaphase transition

Disease: Mosaic Variegated Aneuploidy Syndrome 1; Premature Chromatid Separation Trait

NCBI Summary:This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]
UniProt Code:O60566
NCBI GenInfo Identifier:143811368
NCBI Gene ID:701
NCBI Accession:O60566.3
UniProt Secondary Accession:O60566,O60501, O60627, O60758, O75389, Q59HH6, Q8WV50 Q96KM4, B2R6U0, B4DL09, B4DLG3,
UniProt Related Accession:O60566
Molecular Weight:1050
NCBI Full Name:Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
NCBI Synonym Full Names:BUB1 mitotic checkpoint serine/threonine kinase B
NCBI Official Symbol:BUB1B  
NCBI Official Synonym Symbols:MVA1; SSK1; BUBR1; Bub1A; MAD3L; hBUBR1; BUB1beta  
NCBI Protein Information:mitotic checkpoint serine/threonine-protein kinase BUB1 beta; mitotic checkpoint kinase MAD3L; MAD3/BUB1-related protein kinase; budding uninhibited by benzimidazoles 1 homolog beta
UniProt Protein Name:Mitotic checkpoint serine/threonine-protein kinase BUB1 beta
UniProt Synonym Protein Names:MAD3/BUB1-related protein kinase; hBUBR1; Mitotic checkpoint kinase MAD3L; Protein SSK1
Protein Family:Mitotic checkpoint serine/threonine-protein kinase
UniProt Gene Name:BUB1B  
UniProt Entry Name:BUB1B_HUMAN
Anti-BUB1B Antibody (CAB14525)Western blot analysis of extracts of various cell lines, using BUB1B antibody (CAB14525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
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