Cell Death Antibodies 1

Anti-BUB1 Antibody (CAB1929)

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SKU:
CAB1929
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

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Antibody Name:Anti-BUB1 Antibody
Antibody SKU:CAB1929
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_004327.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HepG2, MCF7, HeLa, SW480, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_004327.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDTP ENVL QMLE AHMQ SYKG NDPL GEWE RYIQ WVEE NFPE NKEY LITL LEHL MKEF LDKK KYHN DPRF ISYC LKFA EYNS DLHQ FFEF LYNH GIGT LSSP LYIA WAGH LEAQ GELQ HASA VLQR GIQN QAEP REFL QQQY RLFQ TRLT ETHL PAQA RTSE PLHN VQVL NQMI TSKS NPGN NMAC ISKN QGSE LSGV ISSA CDKE SNME RRVI TISK SEYS VHSS LASK VDVE QVVM YCKE KLIR GESE FSFE ELRA QKYN QRRK HEQW VNED RHYM KRKE ANAF EEQL LKQK MDEL HKKL
Gene ID:699
Uniprot:O43683
Cellular Location:Chromosome, Nucleus, centromere, kinetochore
Calculated MW:115kDa/120kDa/122kDa
Observed MW:133kDa
Synonyms:BUB1, BUB1A, BUB1L, hBUB1
Background:This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:BUB1: a mitotic-checkpoint protein kinase of the BUB family. Autophosphorylated when the cells enters mitosis. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage. High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index. LOF mutations seen in two colon cancer cell lines exhibiting chromosomal instability, in several leukemias and lymphomas, and in a pancreatic cancer cell line. BUB1 functions in mitotic spindle assembly, and may cause chromosomal instability. In mouse BRCA2 knockouts, mutations in BUB1 and BUBR1 are frequently seen in the resulting tumors. BUB1 and BUBR1 are overexpressed, but not mutated, in many gastric cancers.
UniProt Protein Details:

Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); Cell cycle regulation; Kinase, protein; EC 2.7.11.1; Other group; BUB family

Chromosomal Location of Human Ortholog: 2q14

Cellular Component: nucleoplasm; kinetochore; membrane; cytoplasm; cytosol; condensed nuclear chromosome, pericentric region

Molecular Function:protein serine/threonine kinase activity; protein binding; ATP binding; protein kinase activity

Biological Process: mitosis; cell proliferation; viral reproduction; apoptosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; regulation of sister chromatid cohesion; regulation of chromosome segregation; mitotic cell cycle; protein amino acid phosphorylation; chromosome segregation

NCBI Summary:This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
UniProt Code:O43683
NCBI GenInfo Identifier:8134347
NCBI Gene ID:699
NCBI Accession:O43683.1
UniProt Secondary Accession:O43683,O43430, O43643, O60626, Q53QE4, E9PC26, F5GXI5
UniProt Related Accession:O43683
Molecular Weight:120,002 Da
NCBI Full Name:Mitotic checkpoint serine/threonine-protein kinase BUB1
NCBI Synonym Full Names:BUB1 mitotic checkpoint serine/threonine kinase
NCBI Official Symbol:BUB1  
NCBI Official Synonym Symbols:BUB1A; BUB1L; hBUB1  
NCBI Protein Information:mitotic checkpoint serine/threonine-protein kinase BUB1; mitotic spindle checkpoint kinase; putative serine/threonine-protein kinase; budding uninhibited by benzimidazoles 1 homolog; BUB1 budding uninhibited by benzimidazoles 1 homolog
UniProt Protein Name:Mitotic checkpoint serine/threonine-protein kinase BUB1
UniProt Synonym Protein Names:BUB1A
Protein Family:Checkpoint serine/threonine-protein kinase
UniProt Gene Name:BUB1  
UniProt Entry Name:BUB1_HUMAN
Anti-BUB1 Antibody (CAB1929)Western blot analysis of extracts of various cell lines, using BUB1 antibody (CAB1929) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-BUB1 Antibody (CAB1929)Immunohistochemistry of paraffin-embedded rat liver using BUB1 Antibody (CAB1929) at dilution of 1:200 (40x lens).
Anti-BUB1 Antibody (CAB1929)Immunohistochemistry of paraffin-embedded mouse testis using BUB1 Antibody (CAB1929) at dilution of 1:200 (40x lens).
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