Immunology Antibodies 1

Anti-BTN3A3 Antibody (CAB14269)

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SKU:
CAB14269
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-BTN3A3 Antibody
Antibody SKU:CAB14269
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 510-584 of human BTN3A3 (NP_008925.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Raji, Jurkat, Mouse spleen, Mouse liver, Rat spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 510-584 of human BTN3A3 (NP_008925.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TICP IPKE VESS PDPD LVPD HSLE TPLT PGLA NESG EPQA EVTS LLLP AHPG AEVS PSAT TNQN HKLQ ARTE ALY
Gene ID:10384
Uniprot:O00478
Cellular Location:Cell membrane, Single-pass type I membrane protein
Calculated MW:59kDa/65kDa
Observed MW:65kDa
Synonyms:BTN3A3, BTF3, BTN3.3
Background:
UniProt Protein Function:BTN3A3: Plays a role in T-cell responses in the adaptive immune response. Belongs to the immunoglobulin superfamily. BTN/MOG family. Homodimer
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: cytoplasm; Golgi apparatus; membrane; nucleolus; plasma membrane

Biological Process: T cell mediated immunity

NCBI Summary:The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene (MIM 601610) and the BTN2 (e.g., BTN2A1; MIM 613590) and BTN3 (e.g., BNT3A3) genes, which have undergone tandem duplication, resulting in 3 copies of each (summary by Smith et al., 2010 [PubMed 20208008]).[supplied by OMIM, Nov 2010]
UniProt Code:O00478
NCBI GenInfo Identifier:59799178
NCBI Gene ID:10384
NCBI Accession:O00478.1
UniProt Secondary Accession:O00478,B4DWI7, E9PCP5,
UniProt Related Accession:O00478
Molecular Weight:59,688 Da
NCBI Full Name:Butyrophilin subfamily 3 member A3
NCBI Synonym Full Names:butyrophilin subfamily 3 member A3
NCBI Official Symbol:BTN3A3  
NCBI Official Synonym Symbols:BTF3; BTN3.3  
NCBI Protein Information:butyrophilin subfamily 3 member A3
UniProt Protein Name:Butyrophilin subfamily 3 member A3
Protein Family:Butyrophilin subfamily
UniProt Gene Name:BTN3A3  
UniProt Entry Name:BT3A3_HUMAN
Anti-BTN3A3 Antibody (CAB14269)Western blot analysis of extracts of various cell lines, using BTN3A3 antibody (CAB14269) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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