Cell Biology Antibodies 11
Anti-BSND Antibody (CAB7747)
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- SKU:
- CAB7747
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-BSND Antibody |
| Antibody SKU: | CAB7747 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 54-320 of human BSND (NP_476517.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, Rat liver, Rat kidney, Rat intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 54-320 of human BSND (NP_476517.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CQCY PKIT FVPA DSDF QGIL SPKA MGLL ENGL AAEM KSPS PQPP YVRL WEEA AYDQ SLPD FSHI QMKV MSYS EDHR SLLA PEMG QPKL GTSD GGEG GPGD VQAW MEAA VVIH KGSD ESEG ERRL TQSW PGPL ACPQ GPAP LASF QDDL DMDS SEGS SPNA SPHD REEA CSPQ QEPQ GCRC PLDR FQDF ALID APTL EDEP QEGQ QWEI ALPN NWQR YPRT KVEE KEAS DTGG EEPE KEEE DLYY GLPD GAGD LLPD KELG FEPD TQG |
| Gene ID: | 7809 |
| Uniprot: | Q8WZ55 |
| Cellular Location: | Cell membrane, Cytoplasm, Multi-pass membrane protein |
| Calculated MW: | 35kDa |
| Observed MW: | 35kDa |
| Synonyms: | BSND, BART, DFNB73, barttin |
| Background: | This gene encodes an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear. Mutations in this gene have been associated with Bartter syndrome with sensorineural deafness. |
| UniProt Protein Function: | BSND: Functions as a beta-subunit for CLCNKA and CLCNKB chloride channels. In the kidney CLCNK/BSND heteromers mediate chloride reabsorption by facilitating its basolateral efflux. In the stria, CLCNK/BSND channels drive potassium secretion by recycling chloride for the basolateral SLC12A2 cotransporter. Defects in BSND are the cause of Bartter syndrome type 4A (BS4A); also known as infantile Bartter syndrome with sensorineural deafness. BS refers to a group of autosomal recessive disorders characterized by impaired salt reabsorption in the thick ascending loop of Henle with pronounced salt wasting, hypokalemic metabolic alkalosis, and varying degrees of hypercalciuria. BS4A is associated with sensorineural deafness. |
| UniProt Protein Details: | Protein type:Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, ion channel Chromosomal Location of Human Ortholog: 1p32.1 Cellular Component: basolateral plasma membrane; integral to plasma membrane; plasma membrane; protein complex Molecular Function:chloride channel activity; voltage-gated chloride channel activity Disease: Bartter Syndrome, Type 4a |
| NCBI Summary: | This gene encodes an essential beta subunit for CLC chloride channels. These heteromeric channels localize to basolateral membranes of renal tubules and of potassium-secreting epithelia of the inner ear. Mutations in this gene have been associated with Bartter syndrome with sensorineural deafness. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8WZ55 |
| NCBI GenInfo Identifier: | 54035724 |
| NCBI Gene ID: | 7809 |
| NCBI Accession: | Q8WZ55.1 |
| UniProt Secondary Accession: | Q8WZ55,Q6NT28, |
| UniProt Related Accession: | Q8WZ55 |
| Molecular Weight: | 35,197 Da |
| NCBI Full Name: | Barttin |
| NCBI Synonym Full Names: | barttin CLCNK type accessory beta subunit |
| NCBI Official Symbol: | BSND |
| NCBI Official Synonym Symbols: | BART; DFNB73 |
| NCBI Protein Information: | barttin |
| UniProt Protein Name: | Barttin |
| Protein Family: | Barttin |
| UniProt Gene Name: | BSND |
| UniProt Entry Name: | BSND_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BSND antibody (CAB7747) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using BSND antibody (CAB7747) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using BSND antibody (CAB7747) at dilution of 1:100 (40x lens). |
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