Cell Biology Antibodies 5
Anti-BRIX1 Antibody (CAB14481)
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- SKU:
- CAB14481
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-BRIX1 Antibody |
| Antibody SKU: | CAB14481 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human BRIX1 (NP_060791.3). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-87MG, HeLa, A-549, Mouse spleen, Mouse testis, Rat spleen, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-353 of human BRIX1 (NP_060791.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAT KRKR RGGF AVQA KKPK RNEI DAEP PAKR HATA EEVE EEER DRIP GPVC KGKW KNKE RILI FSSR GINF RTRH LMQD LRML MPHS KADT KMDR KDKL FVIN EVCE MKNC NKCI YFEA KKKQ DLYM WLSN SPHG PSAK FLVQ NIHT LAEL KMTG NCLK GSRP LLSF DPAF DELP HYAL LKEL LIQI FSTP RYHP KSQP FVDH VFTF TILD NRIW FRNF QIIE EDAA LVEI GPRF VLNL IKIF QGSF GGPT LYEN PHYQ SPNM HRRV IRSI TAAK YREK QQVK DVQK LRKK EPKT LLPH DPTA DVFV TPAE EKPI EIQW VKPE PKVD LKAR KKRI YKRQ RKMK QRMD SGKT K |
| Gene ID: | 55299 |
| Uniprot: | Q8TDN6 |
| Cellular Location: | Nucleus, nucleolus |
| Calculated MW: | 41kDa |
| Observed MW: | 41kDa |
| Synonyms: | BRIX1, BRIX, BXDC2, BRX1 |
| Background: |
| UniProt Protein Function: | BXDC2: Required for biogenesis of the 60S ribosomal subunit. Belongs to the BRX1 family. |
| UniProt Protein Details: | Protein type:Ligase; RNA-binding; Nucleolus Chromosomal Location of Human Ortholog: 5p13.2 Cellular Component: nucleolus; nucleus Molecular Function:RNA binding Biological Process: ribosomal large subunit assembly and maintenance |
| UniProt Code: | Q8TDN6 |
| NCBI GenInfo Identifier: | 30580352 |
| NCBI Gene ID: | 55299 |
| NCBI Accession: | Q8TDN6.2 |
| UniProt Secondary Accession: | Q8TDN6,Q3ZTT4, Q8N453, Q96DH1, A8K0P5, |
| UniProt Related Accession: | Q8TDN6 |
| Molecular Weight: | 41,401 Da |
| NCBI Full Name: | Ribosome biogenesis protein BRX1 homolog |
| NCBI Synonym Full Names: | BRX1, biogenesis of ribosomes |
| NCBI Official Symbol: | BRIX1 |
| NCBI Official Synonym Symbols: | BRIX; BXDC2 |
| NCBI Protein Information: | ribosome biogenesis protein BRX1 homolog |
| UniProt Protein Name: | Ribosome biogenesis protein BRX1 homolog |
| UniProt Synonym Protein Names: | Brix domain-containing protein 2 |
| UniProt Gene Name: | BRIX1 |
| UniProt Entry Name: | BRX1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BRIX1 antibody (CAB14481) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using BRIX1 antibody (CAB14481) at dilution of 1:100 (40x lens). |
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