Epigenetics & Nuclear Signaling Antibodies 4

Anti-BRIP1 Antibody (CAB6804)

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SKU:
CAB6804
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-BRIP1 Antibody
Antibody SKU:CAB6804
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BRIP1 (NP_114432.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:Jurkat, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BRIP1 (NP_114432.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSM WSEY TIGG VKIY FPYK AYPS QLAM MNSI LRGL NSKQ HCLL ESPT GSGK SLAL LCSA LAWQ QSLS GKPA DEGV SEKA EVQL SCCC ACHS KDFT NNDM NQGT SRHF NYPS TPPS ERNG TSST CQDS PEKT TLAA KLSA KKQA SIYR DEND DFQV EKKR IRPL ETTQ QIRK RHCF GTEV HNLD AKVD SGKT VKLN SPLE KINS FSPQ KPPG HCSR CCCS TKQG NSQE SSNT IKKD HTGK SKIP KIYF GTRT HKQI AQIT
Gene ID:83990
Uniprot:Q9BX63
Cellular Location:Nucleus
Calculated MW:112kDa/140kDa
Observed MW:145kDa
Synonyms:BRIP1, BACH1, FANCJ, OF
Background:The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations.
UniProt Protein Function:BRIP1: a DNA-dependent ATPase and DNA helicase required for the maintenance of chromosomal stability. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1. Binds directly to the BRCT domains of BRCA1. Defects in BRIP1 cause of susceptibility to breast cancer and Fanconi anemia. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Belongs to the DEAD box helicase family, DEAH subfamily. Two alternatively spliced human isoforms have been described.
UniProt Protein Details:

Protein type:Oncoprotein; Helicase; EC 3.6.4.13

Chromosomal Location of Human Ortholog: 17q22.2

Cellular Component: nuclear membrane; cytoplasm; nucleus

Molecular Function:ATP-dependent DNA helicase activity; protein binding; DNA binding; 4 iron, 4 sulfur cluster binding; metal ion binding; ATP binding

Biological Process: regulation of transcription from RNA polymerase II promoter; double-strand break repair; DNA damage checkpoint; DNA duplex unwinding

Disease: Fanconi Anemia, Complementation Group J; Breast Cancer; Tracheoesophageal Fistula With Or Without Esophageal Atresia

NCBI Summary:The protein encoded by this gene is a member of the RecQ DEAH helicase family and interacts with the BRCT repeats of breast cancer, type 1 (BRCA1). The bound complex is important in the normal double-strand break repair function of breast cancer, type 1 (BRCA1). This gene may be a target of germline cancer-inducing mutations. [provided by RefSeq, Jul 2008]
UniProt Code:Q9BX63
NCBI GenInfo Identifier:57012613
NCBI Gene ID:83990
NCBI Accession:Q9BX63.1
UniProt Secondary Accession:Q9BX63,Q3MJE2, Q8NCI5,
UniProt Related Accession:Q9BX63
Molecular Weight:1249
NCBI Full Name:Fanconi anemia group J protein
NCBI Synonym Full Names:BRCA1 interacting protein C-terminal helicase 1
NCBI Official Symbol:BRIP1  
NCBI Official Synonym Symbols:OF; BACH1; FANCJ  
NCBI Protein Information:Fanconi anemia group J protein; ATP-dependent RNA helicase BRIP1; BRCA1/BRCA2-associated helicase 1; BRCA1-associated C-terminal helicase 1; BRCA1-binding helicase-like protein BACH1
UniProt Protein Name:Fanconi anemia group J protein
UniProt Synonym Protein Names:ATP-dependent RNA helicase BRIP1; BRCA1-associated C-terminal helicase 1; BRCA1-interacting protein C-terminal helicase 1; BRCA1-interacting protein 1
Protein Family:Fanconi anemia group J protein
UniProt Gene Name:BRIP1  
UniProt Entry Name:FANCJ_HUMAN
Anti-BRIP1 Antibody (CAB6804)Western blot analysis of extracts of various cell lines, using BRIP1 antibody (CAB6804) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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