Metabolism Antibodies 3
Anti-BRF2 Antibody (CAB8782)
- SKU:
- CAB8782
- Product Type:
- Antibody
- Applications:
- WB
- Applications:
- IHC
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-BRF2 Antibody |
| Antibody SKU: | CAB8782 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human BRF2 (NP_060780.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | DU145, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human BRF2 (NP_060780.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPGR GRCP DCGS TELV EDSH YSQS QLVC SDCG CVVT EGVL TTTF SDEG NLRE VTYS RSTG ENEQ VSRS QQRG LRRV RDLC RVLQ LPPT FEDT AVAY YQQA YRHS GIRA ARLQ KKEV LVGC |
| Gene ID: | 55290 |
| Uniprot: | Q9HAW0 |
| Cellular Location: | Nucleus |
| Calculated MW: | 44kDa/46kDa |
| Observed MW: | 60kDa |
| Synonyms: | BRF2, BRFU, TFIIIB50 |
| Background: | This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. |
| UniProt Protein Function: | TFIIIB50: General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. Belongs to the TFIIB family. |
| UniProt Protein Details: | Protein type:Transcription regulation Chromosomal Location of Human Ortholog: 8p11.23 Cellular Component: nucleoplasm Molecular Function:zinc ion binding Biological Process: transcription from RNA polymerase III promoter; regulation of transcription, DNA-dependent; transcription initiation; gene expression |
| NCBI Summary: | This gene encodes one of the multiple subunits of the RNA polymerase III transcription factor complex required for transcription of genes with promoter elements upstream of the initiation site. The product of this gene, a TFIIB-like factor, is directly recruited to the TATA-box of polymerase III small nuclear RNA gene promoters through its interaction with the TATA-binding protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9HAW0 |
| NCBI GenInfo Identifier: | 22035562 |
| NCBI Gene ID: | 55290 |
| NCBI Accession: | NP_060780.2 |
| UniProt Secondary Accession: | Q9HAW0,Q9H2Y3, Q9H3B3, Q9NUY6, B2RD62, D3DSW6, |
| UniProt Related Accession: | Q9HAW0 |
| Molecular Weight: | 46,533 Da |
| NCBI Full Name: | transcription factor IIIB 50 kDa subunit |
| NCBI Synonym Full Names: | BRF2, RNA polymerase III transcription initiation factor 50 kDa subunit |
| NCBI Official Symbol: | BRF2 |
| NCBI Official Synonym Symbols: | BRFU; TFIIIB50 |
| NCBI Protein Information: | transcription factor IIIB 50 kDa subunit; BRF-2; hBRFU; hTFIIIB50; B-related factor 2; transcription factor IIB- related factor, TFIIIB50; RNA polymerase III transcription initiation factor BRFU; BRF2, subunit of RNA polymerase III transcription initiation factor, BRF1-like |
| UniProt Protein Name: | Transcription factor IIIB 50 kDa subunit |
| UniProt Synonym Protein Names: | B-related factor 2; BRF-2; hBRFU |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | BRF2 |
| UniProt Entry Name: | BRF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BRF2 antibody (CAB8782) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
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