Signal Transduction Antibodies 2
Anti-BRAP Antibody (CAB16451)
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- CAB16451
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Description
| Antibody Name: | Anti-BRAP Antibody |
| Antibody SKU: | CAB16451 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BRAP (NP_006759.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | U-251MG, HepG2, MCF7, mouse brain, rat testis, rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BRAP (NP_006759.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSVS LVVI RLEL AEHS PVPA GFGF SAAA GEMS DEEI KKTT LASA VACL EGKS PGEK VAII HQHL GRRE MTDV IIET MKSN PDEL KTTV EERK SSEA SPTA QRSK DHSK ECIN AAPD SPSK QLPD QISF FSGN PSVE IVHG IMHL YKTN KMTS LKED VRRS AMLC ILTV PAAM TSHD LMKF VAPF NEVI EQMK IIRD STPN |
| Gene ID: | 8315 |
| Uniprot: | Q7Z569 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 47kDa/67kDa |
| Observed MW: | 72kDa |
| Synonyms: | BRAP, BRAP2, IMP, RNF52 |
| Background: | The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. |
| UniProt Protein Function: | BRAP: Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto- polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport. |
| UniProt Protein Details: | Protein type:Ubiquitin ligase; EC 6.3.2.19; EC 6.3.2.-; Ubiquitin conjugating system; Ligase Chromosomal Location of Human Ortholog: 12q24 Cellular Component: cytoplasm; cytosol; nuclear membrane; ubiquitin ligase complex Molecular Function:identical protein binding; nuclear localization sequence binding; protein binding; ubiquitin binding; ubiquitin-protein ligase activity Biological Process: MAPKKK cascade; negative regulation of signal transduction; protein ubiquitination; Ras protein signal transduction |
| NCBI Summary: | The protein encoded by this gene was identified by its ability to bind to the nuclear localization signal of BRCA1 and other proteins. It is a cytoplasmic protein which may regulate nuclear targeting by retaining proteins with a nuclear localization signal in the cytoplasm. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q7Z569 |
| NCBI GenInfo Identifier: | 296434410 |
| NCBI Gene ID: | 8315 |
| NCBI Accession: | Q7Z569.2 |
| UniProt Secondary Accession: | Q7Z569,O43238, O75341, B4DRM1, B9EGS8, |
| UniProt Related Accession: | Q7Z569 |
| Molecular Weight: | 47,853 Da |
| NCBI Full Name: | BRCA1-associated protein |
| NCBI Synonym Full Names: | BRCA1 associated protein |
| NCBI Official Symbol: | BRAP |
| NCBI Official Synonym Symbols: | IMP; BRAP2; RNF52 |
| NCBI Protein Information: | BRCA1-associated protein |
| UniProt Protein Name: | BRCA1-associated protein |
| UniProt Synonym Protein Names: | BRAP2; Impedes mitogenic signal propagation; IMP; RING finger protein 52; Renal carcinoma antigen NY-REN-63 |
| UniProt Gene Name: | BRAP |
| UniProt Entry Name: | BRAP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BRAP antibody (CAB16451) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human lung cancer using BRAP Rabbit pAb (CAB16451) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using BRAP Rabbit pAb (CAB16451) at dilution of 1:100 (40x lens). |
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Additional Information
Product Type: |
Antibody |
Reactivity: |
Human |
Reactivity: |
Mouse |
Reactivity: |
Rat |
Host Species: |
Rabbit |
Isotype: |
IgG |
Antibody Type: |
Polyclonal Antibody |
Research Area: |
Signal Transduction |
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