Cell Biology Antibodies 1
Anti-BRAF Antibody (CAB0038)
- SKU:
- CAB0038
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-BRAF Antibody |
| Antibody SKU: | CAB0038 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 280-460 of human BRAF (NP_004324.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HepG2, Jurkat, Mouse brain, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 280-460 of human BRAF (NP_004324.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | CVNY DQLD LLFV SKFF EHHP IPQE EASL AETA LTSG SSPS APAS DSIG PQIL TSPS PSKS IPIP QPFR PADE DHRN QFGQ RDRS SSAP NVHI NTIE PVNI DDLI RDQG FRGD GGST TGLS ATPP ASLP GSLT NVKA LQKS PGPQ RERK SSSS SEDR NRMK TLGR RDSS DDWE IPDG QITV G |
| Gene ID: | 673 |
| Uniprot: | P15056 |
| Cellular Location: | Cell membrane, Cytoplasm, Nucleus |
| Calculated MW: | 84kDa |
| Observed MW: | 98kDa |
| Synonyms: | BRAF, B-RAF1, B-raf, BRAF1, NS7, RAFB1 |
| Background: | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. |
| UniProt Protein Function: | BRAF: a tyrosine kinase-like kinase of the RAF family. Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Frequently mutated in thyroid cancers, skin melanomas and at lower frequency in a wide range of human cancers. An activating mutation, mimicking phosphorylation of the activation loop, is seen in 60% of malignant melanoma samples. Raf mutations are generally exclusive to Ras activating mutations. Activating mutations are also seen in ~10% of colorectal cancers, in lung cancers and gliomas, and at a lower rate in several other tumors. Inactivating mutations are also seen and may result in activation of c-Raf and Erk. Mutations in B-Raf, MEK1 and MEK2 also associated with cardiofaciocutaneous syndrome, displaying morphological, cardiac and mental defects. Approved Inhibitor: Nexavar/Sorafenib. |
| UniProt Protein Details: | Protein type:Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; Oncoprotein; EC 2.7.11.1; TKL group; RAF family Chromosomal Location of Human Ortholog: 7q34 Cellular Component: neuron projection; mitochondrion; plasma membrane; cytosol; nucleus Molecular Function:small GTPase binding; protein serine/threonine kinase activity; identical protein binding; protein binding; MAP kinase kinase kinase activity; mitogen-activated protein kinase kinase binding; protein heterodimerization activity; calcium ion binding; ATP binding; protein kinase activity Biological Process: positive T cell selection; response to peptide hormone stimulus; fibroblast growth factor receptor signaling pathway; response to cAMP; CD4-positive, alpha beta T cell differentiation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; somatic stem cell maintenance; protein heterooligomerization; MAPKKK cascade; protein amino acid phosphorylation; positive regulation of peptidyl-serine phosphorylation; regulation of cell proliferation; synaptic transmission; organ morphogenesis; myeloid progenitor cell differentiation; small GTPase mediated signal transduction; positive regulation of stress fiber formation; visual learning; negative regulation of neuron apoptosis; negative regulation of apoptosis Disease: Noonan Syndrome 1; Noonan Syndrome 7; Lung Cancer; Cardiofaciocutaneous Syndrome 1; Leopard Syndrome 3 |
| NCBI Summary: | This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P15056 |
| NCBI GenInfo Identifier: | 50403720 |
| NCBI Gene ID: | 673 |
| NCBI Accession: | P15056.4 |
| UniProt Secondary Accession: | P15056,Q13878, Q3MIN6, Q9UDP8, Q9Y6T3, A4D1T4, B6HY61 B6HY62, B6HY63, B6HY64, B6HY65, B6HY66, |
| UniProt Related Accession: | P15056 |
| Molecular Weight: | 84kDa |
| NCBI Full Name: | Serine/threonine-protein kinase B-raf |
| NCBI Synonym Full Names: | B-Raf proto-oncogene, serine/threonine kinase |
| NCBI Official Symbol: | BRAF |
| NCBI Official Synonym Symbols: | NS7; BRAF1; RAFB1; B-RAF1 |
| NCBI Protein Information: | serine/threonine-protein kinase B-raf; 94 kDa B-raf protein; proto-oncogene B-Raf; v-raf murine sarcoma viral oncogene homolog B; v-raf murine sarcoma viral oncogene homolog B1; murine sarcoma viral (v-raf) oncogene homolog B1; B-Raf proto-oncogene serine/threonine-protein kinase (p94) |
| UniProt Protein Name: | Serine/threonine-protein kinase B-raf |
| UniProt Synonym Protein Names: | Proto-oncogene B-Raf; p94; v-Raf murine sarcoma viral oncogene homolog B1 |
| Protein Family: | Serine/threonine-protein kinase |
| UniProt Gene Name: | BRAF |
| UniProt Entry Name: | BRAF_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BRAF antibody (CAB0038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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