Antibody Name:	Anti-BNP Antibody
Antibody SKU:	CAB2179
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human BNP (NP_002512.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat heart, mouse pancreas

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human BNP (NP_002512.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MDPQ TAPS RALL LLLF LHLA FLGG RSHP LGSP GSAS DLET SGLQ EQRN HLQG KLSE LQVE QTSL EPLQ ESPR PTGV WKSR EVAT EGIR GHRK MVLY TLRA PRSP KMVQ GSGC FGRK MDRI SSSS GLGC KVLR RH
Gene ID:	4879
Uniprot:	P16860
Cellular Location:	Secreted
Calculated MW:	14kDa
Observed MW:	17kDa

Synonyms:

NPPB, BNP

Background:

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis.

UniProt Protein Function:	NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide; Hormone Chromosomal Location of Human Ortholog: 1p36.2 Cellular Component: extracellular region; extracellular space; protein complex Molecular Function:diuretic hormone activity; hormone activity; protein binding; receptor binding Biological Process: body fluid secretion; cell surface receptor linked signal transduction; cGMP biosynthetic process; negative regulation of angiogenesis; negative regulation of cell growth; receptor guanylyl cyclase signaling pathway; regulation of blood pressure; regulation of vascular permeability; regulation of vasodilation
NCBI Summary:	This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]
UniProt Code:	P16860
NCBI GenInfo Identifier:	113836
NCBI Gene ID:	4879
NCBI Accession:	P16860.1
UniProt Secondary Accession:	P16860,Q6FGY0, Q9P2Q7, B0ZBE9,
UniProt Related Accession:	P16860
Molecular Weight:	14,726 Da
NCBI Full Name:	Natriuretic peptides B
NCBI Synonym Full Names:	natriuretic peptide B
NCBI Official Symbol:	NPPB
NCBI Official Synonym Symbols:	BNP
NCBI Protein Information:	natriuretic peptides B
UniProt Protein Name:	Natriuretic peptides B
UniProt Synonym Protein Names:	Gamma-brain natriuretic peptide
Protein Family:	Brain natriuretic peptide
UniProt Gene Name:	NPPB
UniProt Entry Name:	ANFB_HUMAN

	Western blot analysis of extracts of rat heart, using BNP antibody (CAB2179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Western blot analysis of extracts of mouse pancreas, using BNP antibody (CAB2179) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using BNP Rabbit pAb (CAB2179) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using BNP Rabbit pAb (CAB2179) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of H9C2 cells using BNP Rabbit pAb (CAB2179) at dilution of 1:100. Blue: DAPI for nuclear staining.