Cell Death Antibodies 2

Anti-BNIP3 Antibody (CAB5683)

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SKU:
CAB5683
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-BNIP3 Antibody
Antibody SKU:CAB5683
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, NCI-H460, Jurkat, SH-SY5Y, Mouse kidney, Mouse heart, Mouse liver, Rat brain
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGDA AADP PGPA LPCE FLRP GCGA PLSP GAQL GRGA PTSA FPPP AAEA HPAA RRGL RSPQ LPSG AMSQ NGAP GMQE ESLQ GSWV ELHF SNNG NGGS VPAS VSIY NGDM EKIL LDAQ HESG RSSS KSSH CDSP PRSQ TPQD TNRA SETD THSI GEKN SSQS EEDD
Gene ID:664
Uniprot:Q12983
Cellular Location:Mitochondrion, Mitochondrion outer membrane, Single-pass membrane protein
Calculated MW:27kDa
Observed MW:22-70kDa
Synonyms:BNIP3, NIP3
Background:This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.
UniProt Protein Function:BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family.
UniProt Protein Details:

Protein type:Mitochondrial; Membrane protein, integral

Chromosomal Location of Human Ortholog: 10q26.3

Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; dendrite; cytoplasm; mitochondrial membrane; nuclear envelope; nucleus; integral to mitochondrial outer membrane

Molecular Function:identical protein binding; GTPase binding; protein binding; protein homodimerization activity; protein heterodimerization activity

Biological Process: cell death; positive regulation of protein complex disassembly; induction of apoptosis by granzyme; viral reproduction; positive regulation of apoptosis; apoptosis; neuron apoptosis; positive regulation of programmed cell death; response to hyperoxia; brown fat cell differentiation; autophagic cell death; response to hypoxia; regulation of mitochondrial membrane permeability; mitochondrial fragmentation during apoptosis; negative regulation of membrane potential; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis

NCBI Summary:This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq, Mar 2011]
UniProt Code:Q12983
NCBI GenInfo Identifier:20532402
NCBI Gene ID:664
NCBI Accession:Q12983.2
UniProt Secondary Accession:Q12983,O14620, Q96GP0,
UniProt Related Accession:Q12983
Molecular Weight:21,541 Da
NCBI Full Name:BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
NCBI Synonym Full Names:BCL2/adenovirus E1B 19kDa interacting protein 3
NCBI Official Symbol:BNIP3  
NCBI Official Synonym Symbols:NIP3  
NCBI Protein Information:BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
UniProt Protein Name:BCL2/adenovirus E1B 19 kDa protein-interacting protein 3
Protein Family:BNIP3
UniProt Gene Name:BNIP3  
UniProt Entry Name:BNIP3_HUMAN
Anti-BNIP3 Antibody (CAB5683)Western blot analysis of extracts of various cell lines, using BNIP3 antibody (CAB5683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
Anti-BNIP3 Antibody (CAB5683)Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody (CAB5683) at dilution of 1:100 (40x lens).
Anti-BNIP3 Antibody (CAB5683)Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (CAB5683) at dilution of 1:100 (40x lens).
Anti-BNIP3 Antibody (CAB5683)Immunohistochemistry of paraffin-embedded human liver damage using BNIP3 antibody (CAB5683) at dilution of 1:100 (40x lens).
Anti-BNIP3 Antibody (CAB5683)Immunofluorescence analysis of HeLa cells using BNIP3 antibody (CAB5683) at dilution of 1:100. Blue: DAPI for nuclear staining.
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