Cell Biology Antibodies 1
Anti-BMP2 Antibody (CAB0231)
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- SKU:
- CAB0231
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-BMP2 Antibody |
| Antibody SKU: | CAB0231 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 283-396 of human BMP2 (NP_001191.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, THP-1, PC-12 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 283-396 of human BMP2 (NP_001191.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QAKH KQRK RLKS SCKR HPLY VDFS DVGW NDWI VAPP GYHA FYCH GECP FPLA DHLN STNH AIVQ TLVN SVNS KIPK ACCV PTEL SAIS MLYL DENE KVVL KNYQ DMVV EGCG CR |
| Gene ID: | 650 |
| Uniprot: | P12643 |
| Cellular Location: | Secreted |
| Calculated MW: | 44kDa |
| Observed MW: | 45kDa |
| Synonyms: | BDA2, BMP2A, BMP2 |
| Background: | This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer, which plays a role in bone and cartilage development. Duplication of a regulatory region downstream of this gene causes a form of brachydactyly characterized by a malformed index finger and second toe in human patients. |
| UniProt Protein Function: | Induces cartilage and bone formation (PubMed:3201241). Stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A- ATF4 pathway. BMP2 activation of EIF2AK3 stimulates phosphorylation of EIF2A which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation. In addition stimulates TMEM119, which upregulates the expression of ATF4 (PubMed:24362451). |
| NCBI Summary: | The protein encoded by this gene belongs to the transforming growth factor-beta (TGFB) superfamily. The encoded protein acts as a disulfide-linked homodimer and induces bone and cartilage formation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P12643 |
| NCBI GenInfo Identifier: | 115068 |
| NCBI Gene ID: | 650 |
| NCBI Accession: | P12643.1 |
| UniProt Related Accession: | P12643 |
| Molecular Weight: | |
| NCBI Full Name: | Bone morphogenetic protein 2 |
| NCBI Synonym Full Names: | bone morphogenetic protein 2 |
| NCBI Official Symbol: | BMP2 |
| NCBI Official Synonym Symbols: | BDA2; BMP2A |
| NCBI Protein Information: | bone morphogenetic protein 2; BMP-2A; bone morphogenetic protein 2A |
| UniProt Protein Name: | Bone morphogenetic protein 2 |
| UniProt Synonym Protein Names: | Bone morphogenetic protein 2A; BMP-2A |
| Protein Family: | Bone morphogenetic protein |
| UniProt Gene Name: | BMP2 |
| UniProt Entry Name: | BMP2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BMP2 Antibody (CAB0231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human colon using BMP2 antibody (CAB0231) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using BMP2 antibody (CAB0231) at dilution of 1:200 (40x lens). |
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