Cell Death Antibodies 2

Anti-BMF Antibody (CAB5796)

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SKU:
CAB5796
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-BMF Antibody
Antibody SKU:CAB5796
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human BMF (NP_277038.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:U-937, SW480, Mouse liver, Mouse lung, Mouse spleen, Mouse ovary, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-184 of human BMF (NP_277038.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MEPS QCVE ELED DVFQ PEDG EPVT QPGS LLSA DLFA QSLL DCPL SRLQ LFPL THCC GPGL RPTS QEDK ATQT LSPA SPSQ GVML PCGV TEEP QRLF YGNA GYRL PLPA SFPA VLPI GEQP PEGQ WQHQ AEVQ IARK LQCI ADQF HRLH VQQH QQNQ NRVW WQIL LFLH NLAL NGEE NRNG AGPR
Gene ID:90427
Uniprot:Q96LC9
Cellular Location:
Calculated MW:14kDa/17kDa/20kDa
Observed MW:21kDa
Synonyms:BMF
Background:The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified.
UniProt Protein Function:BMF: May play a role in apoptosis. Isoform 1 seems to be the main initiator. Interacts with MCL1, BCL2, BCL2L1/BCL-Xl, BCL2A1 and BCL2L2/BCL-w. Interacts with the myosin V actin motor complex through its binding to DLC2. Isoform 1 is mainly expressed in B-lymphoid cells. Isoform 2 and isoform 3 are mainly expressed in B-CLL and normal B-cells. Belongs to the Bcl-2 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; Cytoskeletal

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: mitochondrial outer membrane; plasma membrane; acrosome; cytosol; myosin complex

Molecular Function:protein binding

Biological Process: positive regulation of protein homooligomerization; apoptosis; anoikis

NCBI Summary:The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein contains a single BCL2 homology domain 3 (BH3), and has been shown to bind BCL2 proteins and function as an apoptotic activator. This protein is found to be sequestered to myosin V motors by its association with dynein light chain 2, which may be important for sensing intracellular damage and triggering apoptosis. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q96LC9
NCBI GenInfo Identifier:56748563
NCBI Gene ID:90427
NCBI Accession:Q96LC9.1
UniProt Secondary Accession:Q96LC9,Q2M396, Q6NT30, Q6NT56, Q6P9F6, Q7Z7D4, Q7Z7D5 Q9H7K7,
UniProt Related Accession:Q96LC9
Molecular Weight:184
NCBI Full Name:Bcl-2-modifying factor
NCBI Synonym Full Names:Bcl2 modifying factor
NCBI Official Symbol:BMF  
NCBI Protein Information:bcl-2-modifying factor
UniProt Protein Name:Bcl-2-modifying factor
Protein Family:Bcl-2-modifying factor
UniProt Gene Name:BMF  
UniProt Entry Name:BMF_HUMAN
Anti-BMF Antibody (CAB5796)Western blot analysis of extracts of various cell lines, using BMF antibody (CAB5796) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
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