Cell Death Antibodies 2
Anti-BLID Antibody (CAB7275)
- SKU:
- CAB7275
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-BLID Antibody |
| Antibody SKU: | CAB7275 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human BLID (NP_001001786.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | PC-3, A-549, SKOV3, MCF7, Mouse spleen, Rat spleen |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-108 of human BLID (NP_001001786.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MVTL LPIE GQEI HFFE ILES ECVL YTGW IERA SGSS IYPE AKAR LPLE ALLG SNKE PMLP KETV LSLK RYNL GSSA MKRN VPGH VLQR PSYL TRIQ VTLL CNSS AEAL |
| Gene ID: | 414899 |
| Uniprot: | Q8IZY5 |
| Cellular Location: | Cytoplasm, Mitochondrion |
| Calculated MW: | 12kDa |
| Observed MW: | 15kDa |
| Synonyms: | BLID, BRCC2 |
| Background: | This gene encodes a BH3-like motif containing protein involved in cell death. The encoded protein may induce apoptosis in a caspase-dependent manner. The protein is localized in both the cytoplasm and the mitochondrion. |
| UniProt Protein Function: | BLID: Functions as a proapoptotic molecule through the caspase-dependent mitochondrial pathway of cell death. |
| UniProt Protein Details: | Protein type:Mitochondrial; Apoptosis Chromosomal Location of Human Ortholog: 11q24.1 Cellular Component: mitochondrion Biological Process: apoptosis |
| UniProt Code: | Q8IZY5 |
| NCBI GenInfo Identifier: | 158563843 |
| NCBI Gene ID: | |
| NCBI Accession: | Q8IZY5.2 |
| Molecular Weight: | |
| NCBI Full Name: | BH3-like motif-containing cell death inducer |
| UniProt Protein Name: | BH3-like motif-containing cell death inducer |
| UniProt Synonym Protein Names: | Breast cancer cell protein 2 |
| Protein Family: | BH3-like motif-containing cell death inducer |
| UniProt Gene Name: | BLID |
| UniProt Entry Name: | BLID_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BLID antibody (CAB7275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of MCF-7 cells using BLID antibody (CAB7275). Blue: DAPI for nuclear staining. |
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