Cell Death Antibodies 2

Anti-BIRC7 Antibody (CAB5736)

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SKU:
CAB5736
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-BIRC7 Antibody
Antibody SKU:CAB5736
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human BIRC7 (NP_647478.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:MCF7, HL-60, HepG2, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-265 of human BIRC7 (NP_647478.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGPK DSAK CLHR GPQP SHWA AGDG PTQE RCGP RSLG SPVL GLDT CRAW DHVD GQIL GQLR PLTE EEEE EGAG ATLS RGPA FPGM GSEE LRLA SFYD WPLT AEVP PELL AAAG FFHT GHQD KVRC FFCY GGLQ SWKR GDDP WTEH AKWF PSCQ FLLR SKGR DFVH SVQE THSQ LLGS WDPW EEPE DAAP VAPS VPAS GYPE LPTP RREV QSES AQEP GGVS PAEA QRAW WVLE PPGA RDVE AQLR RLQE ERTC KVCL DRAV SIVF V
Gene ID:79444
Uniprot:Q96CA5
Cellular Location:Cytoplasm, Golgi apparatus, Nucleus
Calculated MW:30kDa/32kDa
Observed MW:33kDa
Synonyms:BIRC7, KIAP, LIVIN, ML-IAP, MLIAP, RNF50
Background:This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants
UniProt Protein Function:BIRC7: Apoptotic regulator capable of exerting proapoptotic and anti-apoptotic activities and plays crucial roles in apoptosis, cell proliferation, and cell cycle control. Its anti-apoptotic activity is mediated through the inhibition of CASP3, CASP7 and CASP9, as well as by its E3 ubiquitin-protein ligase activity. As it is a weak caspase inhibitor, its anti-apoptotic activity is thought to be due to its ability to ubiquitinate DIABLO/SMAC targeting it for degradation thereby promoting cell survival. May contribute to caspase inhibition, by blocking the ability of DIABLO/SMAC to disrupt XIAP/BIRC4-caspase interactions. Protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also of MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, inhibits CASP3 and proteolytic activation of pro-CASP9. Isoform 1 blocks staurosporine-induced apoptosis. Isoform 2 blocks etoposide-induced apoptosis. Isoform 2 protects against natural killer (NK) cell killing whereas isoform 1 augments killing. Binds to CASP9. Interaction with DIABLO/SMAC via the BIR domain disrupts binding to CASP9 and apoptotic suppressor activity. Interacts with TAB1. In vitro, interacts with CASP3 and CASP7 via its BIR domain. Isoform 1 and isoform 2 are expressed at very low levels or not detectable in most adult tissues. Detected in adult heart, placenta, lung, lymph node, spleen and ovary, and in several carcinoma cell lines. Isoform 2 is detected in fetal kidney, heart and spleen, and at lower levels in adult brain, skeletal muscle and peripheral blood leukocytes. Belongs to the IAP family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; EC 6.3.2.-; Ligase; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: cytoplasm; Golgi apparatus; nucleus; spindle microtubule

Molecular Function:cysteine protease inhibitor activity; protein binding; ubiquitin-protein ligase activity

Biological Process: negative regulation of apoptosis; protein ubiquitination; regulation of cell proliferation; regulation of signal transduction

NCBI Summary:This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]
UniProt Code:Q96CA5
NCBI GenInfo Identifier:21759008
NCBI Gene ID:79444
NCBI Accession:Q96CA5.2
UniProt Secondary Accession:Q96CA5,Q9BQV0, Q9H2A8, Q9HAP7,
UniProt Related Accession:Q96CA5
Molecular Weight:30,866 Da
NCBI Full Name:Baculoviral IAP repeat-containing protein 7
NCBI Synonym Full Names:baculoviral IAP repeat containing 7
NCBI Official Symbol:BIRC7  
NCBI Official Synonym Symbols:KIAP; LIVIN; MLIAP; RNF50; ML-IAP  
NCBI Protein Information:baculoviral IAP repeat-containing protein 7
UniProt Protein Name:Baculoviral IAP repeat-containing protein 7
UniProt Synonym Protein Names:Kidney inhibitor of apoptosis protein; KIAP
Protein Family:Baculoviral IAP repeat-containing protein
UniProt Gene Name:BIRC7  
Anti-BIRC7 Antibody (CAB5736)Western blot analysis of extracts of various cell lines, using BIRC7 antibody (CAB5736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
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