Description
Antibody Name: | Anti-Biotin Antibody (CAB20684) |
Antibody SKU: | CAB20684 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Other (Wide Range) |
Host Species: | Rabbit |
Immunogen: | Biotin |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Other (Wide Range) |
Positive Samples: | Biotin-Goat-Mouse IgG |
Immunogen: | Biotin |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG1 |
Sequence: | Email for sequence |
Cellular Location: | |
Calculated MW: | |
Observed MW: | 25KDa, 55KDa |
Synonyms: | |
Background: |
Western blot analysis of extracts of various cell lines, using Biotin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s. |