Immunology Antibodies 3

Anti-Bile acid receptor Antibody (CAB8320)

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SKU:
CAB8320
Product Type:
Antibody
Applications:
WB
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Immunology

Description

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Antibody Name:NR1H4 Rabbit Polyclonal Antibody
Antibody SKU:CAB8320
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 207-476 of human NR1H4 (NP_001193908.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:A-549, HeLa, HepG2, 293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 207-476 of human NR1H4 (NP_001193908.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:CKSK RLRK NVKQ HADQ TVNE DSEG RDLR QVTS TTKS CREK TELT PDQQ TLLH FIMD SYNK QRMP QEIT NKIL KEEF SAEE NFLI LTEM ATNH VQVL VEFT KKLP GFQT LDHE DQIA LLKG SAVE AMFL RSAE IFNK KLPS GHSD LLEE RIRN SGIS DEYI TPMF SFYK SIGE LKMT QEEY ALLT AIVI LSPD RQYI KDRE AVEK LQEP LLDV LQKL CKIH QPEN PQHF ACLL GRLT ELRT FNHH HAEM LMSW RVND HKFT PLLC EIWD VQ
Gene ID:9971
Uniprot:Q96RI1
Cellular Location:Nucleus
Calculated MW:48kDa/54kDa/55kDa
Observed MW:59kDa
Synonyms:NR1H4, BAR, FXR, HRR-1, HRR1, PFIC5, RIP14
Background:This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described.
UniProt Protein Function:NR1H4: Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. Heterodimer of NR1H4 and RXR. After activation by agonist binding, interacts with a coactivator, NCOA1 or NCOA2. Interacts with CARM1 and SMARD1. Belongs to the nuclear hormone receptor family. NR1 subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q23.1

Cellular Component: nucleoplasm

Molecular Function:bile acid binding; ligand-dependent nuclear receptor activity; ligand-dependent nuclear receptor binding; protein binding; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription coactivator activity; transcription corepressor activity; transcription factor activity

Biological Process: bile acid and bile salt transport; intracellular receptor-mediated signaling pathway; negative regulation of apoptosis; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; signal transduction; toll-like receptor 4 signaling pathway; transcription initiation from RNA polymerase II promoter

NCBI Summary:This gene encodes a ligand-activated transcription factor that shares structural features in common with nuclear hormone receptor family members. This protein functions as a receptor for bile acids, and when bound to bile acids, binds to DNA and regulates the expression of genes involved in bile acid synthesis and transport. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Feb 2016]
UniProt Code:Q96RI1
NCBI GenInfo Identifier:46577705
NCBI Gene ID:9971
NCBI Accession:Q96RI1.2
UniProt Secondary Accession:Q96RI1,Q8NFP5, Q8NFP6, Q92943, A1L4K5, B7Z412, B7ZM06 F8VYG8,
UniProt Related Accession:Q96RI1
Molecular Weight:56kDa
NCBI Full Name:Bile acid receptor
NCBI Synonym Full Names:nuclear receptor subfamily 1 group H member 4
NCBI Official Symbol:NR1H4  
NCBI Official Synonym Symbols:BAR; FXR; HRR1; HRR-1; PFIC5; RIP14  
NCBI Protein Information:bile acid receptor
UniProt Protein Name:Bile acid receptor
UniProt Synonym Protein Names:Farnesoid X-activated receptor; Farnesol receptor HRR-1; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor-interacting protein 14; RXR-interacting protein 14
Protein Family:Bile acid receptor
UniProt Gene Name:NR1H4  
Anti-Bile acid receptor Polyclonal Antibody (CAB8320)Western blot analysis of extracts of various cell lines, using NR1H4 antibody (CAB8320) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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