Cell Death Antibodies 1
Anti-Bif-1 Antibody (CAB15158)
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- SKU:
- CAB15158
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-Bif-1 Antibody |
| Antibody SKU: | CAB15158 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-365 of human Bif-1 (NP_057093.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | 293T, U-251MG, A-549, A-431, Mouse brain, Mouse heart, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-365 of human Bif-1 (NP_057093.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KCGE TQKR IGTA DREL IQTS ALNF LTPL RNFI EGDY KTIA KERK LLQN KRLD LDAA KTRL KKAK AAET RNSS EQEL RITQ SEFD RQAE ITRL LLEG ISST HAHH LRCL NDFV EAQM TYYA QCYQ YMLD LQKQ LGSF PSNY LSNN NQTS VTPV PSVL PNAI GSSA MAST SGLV ITSP SNLS DLKE CSGS RKAR VLYD YDAA NSTE LSLL ADEV ITVF SVVG MDSD WLMG ERGN QKGK VPIT YLEL LN |
| Gene ID: | 51100 |
| Uniprot: | Q9Y371 |
| Cellular Location: | Cytoplasm, Cytoplasmic vesicle, Golgi apparatus membrane, Midbody, Mitochondrion outer membrane, Peripheral membrane protein, autophagosome membrane |
| Calculated MW: | 29kDa/40kDa/43kDa |
| Observed MW: | 44kDa |
| Synonyms: | SH3GLB1, Bif-1, CGI-61, PPP1R70, dJ612B15.2 |
| Background: | This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. |
| UniProt Protein Function: | SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Apoptosis; EC 2.3.1.-; Autophagy; Transferase Chromosomal Location of Human Ortholog: 1p22 Cellular Component: cytoplasm; midbody Molecular Function:identical protein binding; protein binding; protein homodimerization activity Biological Process: autophagic cell death; cellular response to amino acid starvation; cellular response to glucose starvation; positive regulation of autophagy; positive regulation of protein oligomerization; protein oligomerization; receptor catabolic process; regulation of cytokinesis; regulation of macroautophagy; regulation of protein stability |
| NCBI Summary: | This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011] |
| UniProt Code: | Q9Y371 |
| NCBI GenInfo Identifier: | 41018158 |
| NCBI Gene ID: | 51100 |
| NCBI Accession: | Q9Y371.1 |
| UniProt Secondary Accession: | Q9Y371,Q5H8U5, Q9H3Z0, Q9NR47, Q9NYA9, B4E182, |
| UniProt Related Accession: | Q9Y371 |
| Molecular Weight: | 29,318 Da |
| NCBI Full Name: | Endophilin-B1 |
| NCBI Synonym Full Names: | SH3 domain containing GRB2 like endophilin B1 |
| NCBI Official Symbol: | SH3GLB1 |
| NCBI Official Synonym Symbols: | Bif-1; CGI-61; PPP1R70; dJ612B15.2 |
| NCBI Protein Information: | endophilin-B1 |
| UniProt Protein Name: | Endophilin-B1 |
| UniProt Synonym Protein Names: | Bax-interacting factor 1; Bif-1; SH3 domain-containing GRB2-like protein B1 |
| Protein Family: | Endophilin |
| UniProt Gene Name: | SH3GLB1 |
| UniProt Entry Name: | SHLB1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Bif-1 antibody (CAB15158) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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