Cell Biology Antibodies 2
Anti-BHMT Antibody (CAB1216)
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- SKU:
- CAB1216
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-BHMT Antibody |
| Antibody SKU: | CAB1216 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 177-406 of human BHMT (NP_001704.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 177-406 of human BHMT (NP_001704.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SGKP VAAT MCIG PEGD LHGV PPGE CAVR LVKA GASI IGVN CHFD PTIS LKTV KLMK EGLE AARL KAHL MSQP LAYH TPDC NKQG FIDL PEFP FGLE PRVA TRWD IQKY AREA YNLG VRYI GGCC GFEP YHIR AIAE ELAP ERGF LPPA SEKH GSWG SGLD MHTK PWVR ARAR KEYW ENLR IASG RPYN PSMS KPDG WGVT KGTA ELMQ QKEA TTEQ QLKE LFEK QKFK SQ |
| Gene ID: | 635 |
| Uniprot: | Q93088 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 44kDa |
| Observed MW: | 45kDa |
| Synonyms: | BHMT, BHMT1, HEL-S-61p |
| Background: | This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. |
| UniProt Protein Function: | BHMT: Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - glycine, serine and threonine; Amino Acid Metabolism - cysteine and methionine; EC 2.1.1.5; Methyltransferase Chromosomal Location of Human Ortholog: 5q14.1 Cellular Component: protein complex; cytoplasm; cytosol Molecular Function:betaine-homocysteine S-methyltransferase activity; homocysteine S-methyltransferase activity; zinc ion binding; protein complex binding Biological Process: S-methylmethionine cycle; protein amino acid methylation; betaine catabolic process; sulfur amino acid metabolic process; S-adenosylmethionine metabolic process; response to organic nitrogen; regulation of homocysteine metabolic process; betaine metabolic process |
| NCBI Summary: | This gene encodes a cytosolic enzyme that catalyzes the conversion of betaine and homocysteine to dimethylglycine and methionine, respectively. Defects in this gene could lead to hyperhomocyst(e)inemia, but such a defect has not yet been observed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q93088 |
| NCBI GenInfo Identifier: | 145559446 |
| NCBI Gene ID: | 635 |
| NCBI Accession: | Q93088.2 |
| UniProt Secondary Accession: | Q93088,O09171, |
| UniProt Related Accession: | Q93088 |
| Molecular Weight: | 45kDa |
| NCBI Full Name: | Betaine--homocysteine S-methyltransferase 1 |
| NCBI Synonym Full Names: | betaine--homocysteine S-methyltransferase |
| NCBI Official Symbol: | BHMT |
| NCBI Official Synonym Symbols: | BHMT1; HEL-S-61p |
| NCBI Protein Information: | betaine--homocysteine S-methyltransferase 1 |
| UniProt Protein Name: | Betaine--homocysteine S-methyltransferase 1 |
| Protein Family: | Betaine--homocysteine S-methyltransferase |
| UniProt Gene Name: | BHMT |
| UniProt Entry Name: | BHMT1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BHMT antibody (CAB1216) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunofluorescence analysis of human liver cancer cells using BHMT antibody (CAB1216) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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