Signal Transduction Antibodies 3

Anti-BGLAP Antibody (CAB6205)

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SKU:
CAB6205
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-BGLAP Antibody
Antibody SKU:CAB6205
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BGLAP (NP_954642.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Rat
Positive Samples:U-87MG, Rat lung
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BGLAP (NP_954642.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MRAL TLLA LLAL AALC IAGQ AGAK PSGA ESSK GAAF VSKQ EGSE VVKR PRRY LYQW LGAP VPYP DPLE PRRE VCEL NPDC DELA DHIG FQEA YRRF YGPV
Gene ID:632
Uniprot:P02818
Cellular Location:Secreted
Calculated MW:10kDa
Observed MW:12KDa
Synonyms:BGLAP, BGP, OC, OCN, osteocalcin
Background:This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product.
UniProt Protein Function:osteocalcin: Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. Belongs to the osteocalcin/matrix Gla protein family.
UniProt Protein Details:

Protein type:Secreted, signal peptide; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: Golgi apparatus; extracellular space; rough endoplasmic reticulum; dendrite; cytoplasm; perikaryon

Molecular Function:structural constituent of bone; hydroxyapatite binding; calcium ion binding; structural molecule activity

Biological Process: response to drug; response to gravity; regulation of bone resorption; response to glucocorticoid stimulus; cell aging; response to testosterone stimulus; bone mineralization; osteoblast development; osteoblast differentiation; odontogenesis; response to vitamin D; regulation of osteoclast differentiation; response to ethanol; response to vitamin K; response to zinc ion; response to mechanical stimulus; response to estrogen stimulus; response to hydroxyisoflavone; response to activity; cell adhesion; skeletal development; regulation of bone mineralization

NCBI Summary:This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
UniProt Code:P02818
NCBI GenInfo Identifier:129253
NCBI Gene ID:632
NCBI Accession:P02818.2
UniProt Secondary Accession:P02818,Q5TCK6,
UniProt Related Accession:P02818
Molecular Weight:10,963 Da
NCBI Full Name:Osteocalcin
NCBI Synonym Full Names:bone gamma-carboxyglutamate (gla) protein
NCBI Official Symbol:BGLAP  
NCBI Official Synonym Symbols:OC; BGP; OCN  
NCBI Protein Information:osteocalcin; bone Gla protein; gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate (gla) protein (osteocalcin)
UniProt Protein Name:Osteocalcin
UniProt Synonym Protein Names:Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein
Protein Family:Osteocalcin
UniProt Gene Name:BGLAP  
UniProt Entry Name:OSTCN_HUMAN
Anti-BGLAP Antibody (CAB6205)Western blot analysis of extracts of U-87MG cells, using BGLAP antibody (CAB6205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-BGLAP Antibody (CAB6205)Western blot analysis of extracts of Rat lung, using BGLAP antibody (CAB6205) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
Anti-BGLAP Antibody (CAB6205)Immunofluorescence analysis of C6 cells using Osteocalcin antibody (CAB6205) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-BGLAP Antibody (CAB6205)Immunofluorescence analysis of U-2 OS cells using Osteocalcin antibody (CAB6205) at dilution of 1:100. Blue: DAPI for nuclear staining.
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