KO Validated Antibodies 1

Anti-BetaIII-Tubulin Antibody (CAB17074)[KO Validated]

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SKU:
CAB17074
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-BetaIII-Tubulin Antibody [KO Validated]
Antibody SKU:CAB17074
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant protein of human BetaIII-Tubulin.
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, MCF7, mouse brain, mouse testis, rat brain, rat testis
Immunogen:Recombinant protein of human BetaIII-Tubulin.
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:10381
Uniprot:Q13509
Cellular Location:Cytoplasm, cytoskeleton
Calculated MW:42kDa/50kDa
Observed MW:55kDa
Synonyms:CDCBM, CDCBM1, CFEOM3, CFEOM3A, FEOM3, TUBB4, beta-4, beta Tubulin III, TUBB3
Background:This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6.
UniProt Protein Function:TUBB3: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance. Dimer of alpha and beta chains. Expression is primarily restricted to central and peripheral nervous system. Greatly increased expression in most cancerous tissues. Belongs to the tubulin family.
UniProt Protein Details:

Protein type:Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: axon; cell soma; cytoplasm; dendrite; microtubule; nucleus

Molecular Function:GTP binding; GTPase activity; peptide binding; protein binding; structural constituent of cytoskeleton

Biological Process: 'de novo' posttranslational protein folding; axon guidance; cellular protein metabolic process; microtubule-based process; mitosis; protein folding

Disease: Cortical Dysplasia, Complex, With Other Brain Malformations 1; Fibrosis Of Extraocular Muscles, Congenital, 3a, With Or Without Extraocular Involvement

NCBI Summary:This gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in this gene are the cause of congenital fibrosis of the extraocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 6. [provided by RefSeq, Oct 2010]
UniProt Code:Q13509
NCBI GenInfo Identifier:20455526
NCBI Gene ID:10381
NCBI Accession:Q13509.2
UniProt Secondary Accession:Q13509,Q9BTZ0, Q9BW10, A8K854,
UniProt Related Accession:Q13509
Molecular Weight:42,433 Da
NCBI Full Name:Tubulin beta-3 chain
NCBI Synonym Full Names:tubulin beta 3 class III
NCBI Official Symbol:TUBB3  
NCBI Official Synonym Symbols:CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A  
NCBI Protein Information:tubulin beta-3 chain
UniProt Protein Name:Tubulin beta-3 chain
UniProt Synonym Protein Names:Tubulin beta-4 chain; Tubulin beta-III
Protein Family:Tubulin
UniProt Gene Name:TUBB3  
UniProt Entry Name:TBB3_HUMAN
Anti-BetaIII-Tubulin Antibody [KO Validated] (CAB17074)Western blot analysis of extracts of various cell lines, using beta III-Tubulin antibody (CAB17074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-BetaIII-Tubulin Antibody [KO Validated] (CAB17074)Western blot analysis of extracts from normal (control) and betaIII-Tubulin Rabbit pAb knockout (KO) HeLa cells, using beta III-Tubulin Rabbit pAb antibody (CAB17074) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-BetaIII-Tubulin Antibody [KO Validated] (CAB17074)Immunofluorescence analysis of mouse brain using beta III-Tubulin antibody (CAB17074) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-BetaIII-Tubulin Antibody [KO Validated] (CAB17074)Immunofluorescence analysis of rat brain using beta III-Tubulin antibody (CAB17074) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-BetaIII-Tubulin Antibody [KO Validated] (CAB17074)Immunofluorescence analysis of U-251MG cells using Beta III-Tubulin antibody (CAB17074) at dilution of 1:100. Blue: DAPI for nuclear staining.
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