KO Validated Antibodies 1

Anti-beta 2 Microglobulin Antibody (CAB12404)[KO Validated]

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SKU:
CAB12404
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Immunology

Description

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Antibody Name:Anti-beta 2 Microglobulin Antibody [KO Validated]
Antibody SKU:CAB12404
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).
Application:WB IHC IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, COS7, HeLa, K-562, Mouse liver, Mouse spleen
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IQRT PKIQ VYSR HPAE NGKS NFLN CYVS GFHP SDIE VDLL KNGE RIEK VEHS DLSF SKDW SFYL LYYT EFTP TEKD EYAC RVNH VTLS QPKI VKWD RDM
Gene ID:567
Uniprot:P61769
Cellular Location:Secreted
Calculated MW:13kDa
Observed MW:12kDa
Synonyms:B2M, IMD43
Background:This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.
UniProt Protein Function:B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.
UniProt Protein Details:

Protein type:Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; early endosome membrane; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; MHC class I protein complex; external side of plasma membrane

Molecular Function:identical protein binding; protein binding

Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent

Disease: Hypoproteinemia, Hypercatabolic

NCBI Summary:This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]
UniProt Code:P61769
NCBI GenInfo Identifier:48428791
NCBI Gene ID:567
NCBI Accession:P61769.1
UniProt Related Accession:P61769
Molecular Weight:
NCBI Full Name:Beta-2-microglobulin
NCBI Synonym Full Names:beta-2-microglobulin
NCBI Official Symbol:B2M  
NCBI Official Synonym Symbols:IMD43  
NCBI Protein Information:beta-2-microglobulin
UniProt Protein Name:Beta-2-microglobulin
Protein Family:Beta-2-microglobulin
UniProt Gene Name:B2M  
UniProt Entry Name:B2MG_HUMAN
Anti-beta 2 Microglobulin Antibody [KO Validated] (CAB12404)Western blot analysis of extracts of various cell lines, using beta 2 Microglobulin antibody (CAB12404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-beta 2 Microglobulin Antibody [KO Validated] (CAB12404)Western blot analysis of extracts from normal (control) and beta 2 Microglobulin knockout (KO) 293T cells, using beta 2 Microglobulin antibody (CAB12404) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-beta 2 Microglobulin Antibody [KO Validated] (CAB12404)Immunohistochemistry of paraffin-embedded rat brain using beta 2 Microglobulin antibody (CAB12404) at dilution of 1:100 (40x lens).
Anti-beta 2 Microglobulin Antibody [KO Validated] (CAB12404)Immunohistochemistry of paraffin-embedded human prostate using beta 2 Microglobulin antibody (CAB12404) at dilution of 1:100 (40x lens).
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