Name: Anti-BDKRB2 Antibody (CAB2844)
Brand: Cell Biology Antibodies 8
SKU: CAB2844
Availability: OutOfStock

Antibody Name:	Anti-BDKRB2 Antibody
Antibody SKU:	CAB2844
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 302-391 of human BDKRB2 (NP_000614.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, MCF7, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 302-391 of human BDKRB2 (NP_000614.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SSCQ DERI IDVI TQIA SFMA YSNS CLNP LVYV IVGK RFRK KSWE VYQG VCQK GGCR SEPI QMEN SMGT LRTS ISVE RQIH KLQD WAGS RQ
Gene ID:	624
Uniprot:	P30411
Cellular Location:	Cell membrane, Multi-pass membrane protein
Calculated MW:	41kDa/44kDa
Observed MW:	44kDa

Synonyms:	BDKRB2, B2R, BK-2, BK2, BKR2, BRB2
Background:	This gene encodes a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. Alternate start codons result in two isoforms of the protein.

UniProt Protein Function:	BKR2: a receptor for bradykinin. Bradykinin elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. Alternate start codons result in two isoforms of the protein.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral Chromosomal Location of Human Ortholog: 14q32.1-q32.2 Cellular Component: integral to plasma membrane; plasma membrane; endosome Molecular Function:bradykinin receptor activity; protein binding; protease binding; protein heterodimerization activity; type 1 angiotensin receptor binding; phosphoinositide phospholipase C activity Biological Process: vasoconstriction; metabolic process; blood circulation; negative regulation of peptidyl-serine phosphorylation; arachidonic acid secretion; vasodilation; response to salt stress; G-protein coupled receptor protein signaling pathway; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; smooth muscle contraction; regulation of vasoconstriction; regulation of vascular permeability; inflammatory response; transmembrane receptor protein tyrosine kinase signaling pathway
NCBI Summary:	This gene encodes a receptor for bradykinin. The 9 aa bradykinin peptide elicits many responses including vasodilation, edema, smooth muscle spasm and pain fiber stimulation. This receptor associates with G proteins that stimulate a phosphatidylinositol-calcium second messenger system. Alternate start codons result in two isoforms of the protein. [provided by RefSeq, Jul 2008]
UniProt Code:	P30411
NCBI GenInfo Identifier:	2506481
NCBI Gene ID:	624
NCBI Accession:	P30411.2
UniProt Related Accession:	P30411
Molecular Weight:
NCBI Full Name:	B2 bradykinin receptor
NCBI Synonym Full Names:	bradykinin receptor B2
NCBI Official Symbol:	BDKRB2
NCBI Official Synonym Symbols:	B2R; BK2; BK-2; BKR2; BRB2
NCBI Protein Information:	B2 bradykinin receptor
UniProt Protein Name:	B2 bradykinin receptor
UniProt Gene Name:	BDKRB2
UniProt Entry Name:	BKRB2_HUMAN

	Western blot analysis of extracts of various cell lines, using BDKRB2 antibody (CAB2844) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunofluorescence analysis of HeLa cells using BDKRB2 Rabbit pAb (CAB2844) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of NIH/3T3 cells using BDKRB2 Rabbit pAb (CAB2844) at dilution of 1:100. Blue: DAPI for nuclear staining.