Anti-Bcl 2 Mouse Antibody (CAB20777)
- SKU:
- CAB20777
- Product Type:
- Antibody
- Antibody Type:
- Secondary Antibody
- Host Species:
- Goat
- Isotype:
- Horseradish peroxidase conjugated IgG
Description
| Antibody Name: | Anti-Bcl 2 Mouse Antibody (CAB20777) |
| Antibody SKU: | CAB20777 |
| Antibody Size: | 50µL, 100µL |
| Application: | Western blotting, Immunofluorescence |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Mouse |
| Immunogen: | Recombinant protein of mouse Bcl-2. |
| Application: | Western blotting, Immunofluorescence |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, Jurkat, 293T, NIH/3T3 |
| Immunogen: | Recombinant protein of mouse Bcl-2. |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Cellular Location: | Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein |
| Calculated MW: | 22kDa/26kDa |
| Observed MW: | 26KDa |
| Synonyms: | Bcl-2, PPP1R50, BCL2 |
| Background: | This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. |
 | Immunofluorescence analysis of PC-12 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Mouse mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Western blot analysis of extracts of various cell lines, using Bcl-2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s. |
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