Cell Biology Antibodies 5
Anti-BCHE Antibody (CAB1460)
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- SKU:
- CAB1460
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-BCHE Antibody |
| Antibody SKU: | CAB1460 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-270 of human BCHE (NP_000046.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, SW620 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 29-270 of human BCHE (NP_000046.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EDDI IIAT KNGK VRGM NLTV FGGT VTAF LGIP YAQP PLGR LRFK KPQS LTKW SDIW NATK YANS CCQN IDQS FPGF HGSE MWNP NTDL SEDC LYLN VWIP APKP KNAT VLIW IYGG GFQT GTSS LHVY DGKF LARV ERVI VVSM NYRV GALG FLAL PGNP EAPG NMGL FDQQ LALQ WVQK NIAA FGGN PKSV TLFG ESAG AASV SLHL LSPG SHSL FTRA ILQS GSFN APWA VTSL YEAR NR |
| Gene ID: | 590 |
| Uniprot: | P06276 |
| Cellular Location: | Secreted |
| Calculated MW: | 68kDa |
| Observed MW: | 68kDa |
| Synonyms: | BCHE, CHE1, CHE2, E1 |
| Background: | Mutant alleles at the BCHE locus are responsible for suxamethonium sensitivity. Homozygous persons sustain prolonged apnea after administration of the muscle relaxant suxamethonium in connection with surgical anesthesia. The activity of pseudocholinesterase in the serum is low and its substrate behavior is atypical. In the absence of the relaxant, the homozygote is at no known disadvantage. |
| UniProt Protein Function: | BCHE: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters. Defects in BCHE are the cause of butyrylcholinesterase deficiency (BChE deficiency). BChE deficiency is a metabolic disorder characterized by prolonged apnoea after the use of certain anesthetic drugs, including the muscle relaxants succinylcholine or mivacurium and other ester local anesthetics. The duration of the prolonged apnoea varies significantly depending on the extent of the enzyme deficiency. BChE deficiency is a multifactorial disorder. The hereditary condition is transmitted as an autosomal recessive trait. Belongs to the type-B carboxylesterase/lipase family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Hydrolase; Nuclear envelope; Secreted; EC 3.1.1.8 Chromosomal Location of Human Ortholog: 3q26.1-q26.2 Cellular Component: membrane; nuclear envelope lumen; endoplasmic reticulum lumen; extracellular region Molecular Function:identical protein binding; choline binding; enzyme binding; cholinesterase activity; beta-amyloid binding; acetylcholinesterase activity; catalytic activity Biological Process: response to drug; response to alkaloid; negative regulation of cell proliferation; choline metabolic process; cocaine metabolic process; cellular protein metabolic process; response to folic acid; response to glucocorticoid stimulus; neuroblast differentiation; learning; negative regulation of synaptic transmission |
| NCBI Summary: | This gene encodes a cholinesterase enzyme and member of the type-B carboxylesterase/lipase family of proteins. The encoded enzyme exhibits broad substrate specificity and is involved in the detoxification of poisons including organophosphate nerve agents and pesticides, and the metabolism of drugs including cocaine, heroin and aspirin. Humans homozygous for certain mutations in this gene exhibit prolonged apnea after administration of the muscle relaxant succinylcholine. [provided by RefSeq, Jul 2016] |
| UniProt Code: | P06276 |
| NCBI GenInfo Identifier: | 116353 |
| NCBI Gene ID: | 590 |
| NCBI Accession: | P06276.1 |
| UniProt Related Accession: | P06276 |
| Molecular Weight: | |
| NCBI Full Name: | Cholinesterase |
| NCBI Synonym Full Names: | butyrylcholinesterase |
| NCBI Official Symbol: | BCHE |
| NCBI Official Synonym Symbols: | E1; CHE1; CHE2; BCHED |
| NCBI Protein Information: | cholinesterase |
| UniProt Protein Name: | Cholinesterase |
| UniProt Synonym Protein Names: | Acylcholine acylhydrolase; Butyrylcholine esterase; Choline esterase II; Pseudocholinesterase |
| Protein Family: | Cholinesterase |
| UniProt Gene Name: | BCHE |
| UniProt Entry Name: | CHLE_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BCHE antibody (CAB1460) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat kidney using BCHE antibody (CAB1460) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using BCHE antibody (CAB1460). Blue: DAPI for nuclear staining. |
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