Antibody Name:	Anti-BCAP31 Antibody
Antibody SKU:	CAB7056
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 127-246 of human BCAP31 (NP_001243376.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	MCF-7, HT-29, SH-SY5Y, HeLa, A-431, Mouse liver, Mouse kidney, Mouse lung, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 127-246 of human BCAP31 (NP_001243376.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ATLL ASNE AFKK QAES ASEA AKKY MEEN DQLK KGAA VDGG KLDV GNAE VKLE EENR SLKA DLQK LKDE LAST KQKL EKAE NQVL AMRK QSEG LTKE YDRL LEEH AKLQ AAVD GPMD KKEE
Gene ID:	10134
Uniprot:	P51572
Cellular Location:	Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Multi-pass membrane protein
Calculated MW:	27kDa/34kDa
Observed MW:	28kDa

Synonyms:

BCAP31, 6C6-AG, BAP31, CDM, DDCH, DXS1357E

Background:

This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16.

UniProt Protein Function:	BCAP31 iso2: May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. Homodimer and heterodimer with BCAP29. Binds CASP8 (isoform 9) as a complex containing BCAP31, BCAP29, BCL2 and/or BCL2L1. Interacts with VAMP3, VAMP1 and membrane IgD immunoglobulins. May interact with ACTG1 and non-muscle myosin II. Interacts with PTPLB. Ubiquitous. Belongs to the BCAP29/BCAP31 family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Membrane protein, multi-pass Chromosomal Location of Human Ortholog: Xq28 Cellular Component: endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; membrane; clathrin-coated vesicle; endoplasmic reticulum; integral to plasma membrane; lipid particle; cytosol Molecular Function:protein binding; MHC class I protein binding; protein complex binding Biological Process: intracellular protein transport; ER to Golgi vesicle-mediated transport; antigen processing and presentation of peptide antigen via MHC class I; elevation of cytosolic calcium ion concentration; apoptosis; reduction of endoplasmic reticulum calcium ion concentration; positive regulation of caspase activity; spermatogenesis; cell structure disassembly during apoptosis; elevation of mitochondrial calcium ion concentration Disease: Deafness, Dystonia, And Cerebral Hypomyelination
NCBI Summary:	This gene encodes a member of the B-cell receptor associated protein 31 superfamily. The encoded protein is a multi-pass transmembrane protein of the endoplasmic reticulum that is involved in the anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi and in caspase 8-mediated apoptosis. Microdeletions in this gene are associated with contiguous ABCD1/DXS1375E deletion syndrome (CADDS), a neonatal disorder. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes have been identified on chromosome 16. [provided by RefSeq, Jan 2012]
UniProt Code:	P51572
NCBI GenInfo Identifier:	1705725
NCBI Gene ID:	10134
NCBI Accession:	P51572.3
UniProt Secondary Accession:	P51572,Q13836, Q96CF0, B3KQ79, D3DWV5,
UniProt Related Accession:	P51572
Molecular Weight:	34,752 Da
NCBI Full Name:	B-cell receptor-associated protein 31
NCBI Synonym Full Names:	B-cell receptor-associated protein 31
NCBI Official Symbol:	BCAP31
NCBI Official Synonym Symbols:	CDM; DDCH; BAP31; 6C6-AG; DXS1357E
NCBI Protein Information:	B-cell receptor-associated protein 31; p28 Bap31; BCR-associated protein Bap31; 6C6-AG tumor-associated antigen
UniProt Protein Name:	B-cell receptor-associated protein 31
UniProt Synonym Protein Names:	6C6-AG tumor-associated antigen; Protein CDM; p28
Protein Family:	B-cell receptor-associated protein
UniProt Gene Name:	BCAP31
UniProt Entry Name:	BAP31_HUMAN

	Western blot analysis of extracts of various cell lines, using BCAP31 antibody (CAB7056) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using BCAP31 antibody (CAB7056). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug BCAP31 antibody (CAB7056). Western blot was performed from the immunoprecipitate using BCAP31 antibody (CAB7056) at a dilition of 1:1000.