Cell Biology Antibodies 7
Anti-BBC3 Antibody (CAB17138)
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- SKU:
- CAB17138
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-BBC3 Antibody |
| Antibody SKU: | CAB17138 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human BBC3 (NP_001120713.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, LO2, HepG2, K562 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50 to the C-terminus of human BBC3 (NP_001120713.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PGAL AGGP TQAA PGVR GEEE QWAR EIGA QLRR MADD LNAQ YERR RQEE QQRH RPSP WRVL YNLI MGLL PLPR GHRA PEME PN |
| Gene ID: | 27113 |
| Uniprot: | Q96PG8/Q9BXH1 |
| Cellular Location: | |
| Calculated MW: | 10kDa/14kDa/20kDa/26kDa |
| Observed MW: | 20kDa |
| Synonyms: | BBC3, JFY-1, JFY1, PUMA |
| Background: | This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | BBC3B: Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity. Induced by DNA damage, glucocorticoid treatment, growth factor deprivation and p53/TP53. {ECO:0000269|PubMed:11463392}. Isoform 3 does not interact with BCL2. 4 isoforms of the human protein are produced by alternative splicing |
| UniProt Protein Details: | Protein type:Apoptosis; Mitochondrial Chromosomal Location of Human Ortholog: 19q13.3-q13.4 Cellular Component: lysosome; mitochondrion Molecular Function:ATPase binding; glycoprotein binding; protein binding Biological Process: apoptosis |
| NCBI Summary: | This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] |
| UniProt Code: | Q96PG8 |
| NCBI GenInfo Identifier: | 391738062 |
| NCBI Gene ID: | 27113 |
| NCBI Accession: | Q96PG8.2 |
| UniProt Related Accession: | Q9BXH1,Q96PG8 |
| Molecular Weight: | 20,532 Da |
| NCBI Full Name: | Bcl-2-binding component 3 |
| NCBI Synonym Full Names: | BCL2 binding component 3 |
| NCBI Official Symbol: | BBC3 |
| NCBI Official Synonym Symbols: | JFY1; PUMA; JFY-1 |
| NCBI Protein Information: | bcl-2-binding component 3 |
| UniProt Protein Name: | Bcl-2-binding component 3 |
| UniProt Synonym Protein Names: | JFY-1; p53 up-regulated modulator of apoptosis |
| Protein Family: | Bcl-2-binding component |
| UniProt Gene Name: | BBC3 |
| UniProt Entry Name: | BBC3B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BBC3 antibody (CAB17138) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of NIH-3T3 cells using BBC3 Polyclonal Antibody (CAB17138) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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