KO Validated Antibodies 1

Anti-Bax Antibody (CAB0207)[KO Validated]

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SKU:
CAB0207
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-Bax Antibody [KO Validated]
Antibody SKU:CAB0207
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 IP 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HT-1080, Raji, 293T
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bax (NP_620116.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MDGS GEQP RGGG PTSS EQIM KTGA LLLQ GFIQ DRAG RMGG EAPE LALD PVPQ DAST KKLS ECLK RIGD ELDS NMEL QRMI AAVD TDSP REVF FRVA ADMF
Gene ID:581
Uniprot:Q07812
Cellular Location:Cytoplasm, Cytoplasm, Mitochondrion membrane, Single-pass membrane protein
Calculated MW:4kDa/12kDa/15kDa/18kDa/19kDa/21kDa/24kDa
Observed MW:21KDa
Synonyms:BCL2L4, BAX
Background:The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
UniProt Protein Function:Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
NCBI Summary:The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q07812
NCBI GenInfo Identifier:728945
NCBI Gene ID:581
NCBI Accession:Q07812.1
UniProt Secondary Accession:Q07812,P55269, Q07814, Q07815, Q8WZ49, Q9NR76, Q9NYG7 Q9UCZ6, Q9UCZ7, Q9UQD6, A8K4W1,
UniProt Related Accession:Q07812
Molecular Weight:19,718 Da
NCBI Full Name:Apoptosis regulator BAX
NCBI Synonym Full Names:BCL2 associated X, apoptosis regulator
NCBI Official Symbol:BAX  
NCBI Official Synonym Symbols:BCL2L4  
NCBI Protein Information:apoptosis regulator BAX
UniProt Protein Name:Apoptosis regulator BAX
UniProt Synonym Protein Names:Bcl-2-like protein 4; Bcl2-L-4
Protein Family:Protein
UniProt Gene Name:BAX  
UniProt Entry Name:BAX_HUMAN
Anti-Bax Antibody [KO Validated] (CAB0207)Western blot analysis of extracts from normal (control) and Bax knockout (KO) 293T cells, using Bax antibody (CAB0207) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-Bax Antibody [KO Validated] (CAB0207)Western blot analysis of extracts of various cell lines, using Bax antibody (CAB0207) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Anti-Bax Antibody [KO Validated] (CAB0207)Immunohistochemistry of paraffin-embedded human esophageal cancer using [KO Validated] Bax Rabbit pAb (CAB0207) at dilution of 1:100 (40x lens).
Anti-Bax Antibody [KO Validated] (CAB0207)Immunofluorescence analysis of NIH/3T3 cells using Bax antibody (CAB0207) at dilution of 1:100. Blue: DAPI for nuclear staining.
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