Cell Death Antibodies 1

Anti-BAG3 Antibody (CAB14826)

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SKU:
CAB14826
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-BAG3 Antibody
Antibody SKU:CAB14826
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).
Application:WB IHC IF IP
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:A-549, HeLa, HepG2, Mouse brain, Mouse testis
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 380-575 of human BAG3 (NP_004272.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PSAV PSSP KSVA TEER AAPS TAPA EATP PKPG EAEA PPKH PGVL KVEA ILEK VQGL EQAV DNFE GKKT DKKY LMIE EYLT KELL ALDS VDPE GRAD VRQA RRDG VRKV QTIL EKLE QKAI DVPG QVQV YELQ PSNL EADQ PLQA IMEM GAVA ADKG KKNA GNAE DPHT ETQQ PEAT AAAT SNPS SMTD TPGN PAAP
Gene ID:9531
Uniprot:O95817
Cellular Location:
Calculated MW:61kDa
Observed MW:80kDa
Synonyms:BAG3, BAG-3, BIS, CAIR-1, MFM6
Background:BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
UniProt Protein Function:BAG3: Inhibits the chaperone activity of HSP70/HSC70 by promoting substrate release. Has anti-apoptotic activity. Defects in BAG3 are the cause of myopathy myofibrillar type 6 (MFM6). A neuromuscular disorder that results in early-onset, severe, progressive, diffuse muscle weakness associated with cardiomyopathy, severe respiratory insufficiency during adolescence, and a rigid spine in some patients. At ultrastructural level, muscle fibers display structural alterations consisting of replacement of the normal myofibrillar markings by small, dense granules, or larger hyaline masses, or amorphous material. Defects in BAG3 are the cause of cardiomyopathy dilated type 1HH (CMD1HH). CMD1HH is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
UniProt Protein Details:

Protein type:Apoptosis

Chromosomal Location of Human Ortholog: 10q26.11

Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; plasma membrane

Molecular Function:adenyl-nucleotide exchange factor activity; protein binding

Biological Process: induction of apoptosis via death domain receptors

Disease: Cardiomyopathy, Dilated, 1hh; Myopathy, Myofibrillar, 6

NCBI Summary:BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq, Jul 2008]
UniProt Code:O95817
NCBI GenInfo Identifier:12643665
NCBI Gene ID:9531
NCBI Accession:O95817.3
UniProt Secondary Accession:O95817,Q3B763, Q9NT20, Q9P120, A8K5L8,
UniProt Related Accession:O95817
Molecular Weight:61,595 Da
NCBI Full Name:BAG family molecular chaperone regulator 3
NCBI Synonym Full Names:BCL2 associated athanogene 3
NCBI Official Symbol:BAG3  
NCBI Official Synonym Symbols:BIS; MFM6; BAG-3; CAIR-1  
NCBI Protein Information:BAG family molecular chaperone regulator 3
UniProt Protein Name:BAG family molecular chaperone regulator 3
UniProt Synonym Protein Names:Bcl-2-associated athanogene 3; Bcl-2-binding protein Bis; Docking protein CAIR-1
Protein Family:BAG family molecular chaperone regulator
UniProt Gene Name:BAG3  
Anti-BAG3 Antibody (CAB14826)Western blot analysis of extracts of various cell lines, using BAG3 antibody (CAB14826) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-BAG3 Antibody (CAB14826)Immunohistochemistry of paraffin-embedded rat heart using BAG3 antibody (CAB14826) at dilution of 1:100 (40x lens).
Anti-BAG3 Antibody (CAB14826)Immunohistochemistry of paraffin-embedded human lung using BAG3 antibody (CAB14826) at dilution of 1:100 (40x lens).
Anti-BAG3 Antibody (CAB14826)Immunohistochemistry of paraffin-embedded mouse heart using BAG3 antibody (CAB14826) at dilution of 1:100 (40x lens).
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