Name: Anti-BAF57/SMARCE1 Antibody (CAB3814)
Brand: Neuroscience
SKU: CAB3814
Availability: OutOfStock

Antibody Name:	Anti-BAF57/SMARCE1 Antibody
Antibody SKU:	CAB3814
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthesized peptide derived from human BAF57/SMARCE1

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse spinal cord, Mouse brain, Rat brain, HeLa, MCF7, Jurkat

Immunogen:	A synthesized peptide derived from human BAF57/SMARCE1
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	6605
Uniprot:	Q969G3
Cellular Location:
Calculated MW:	57kDa
Observed MW:	55KDa

Synonyms:

BAF57, CSS5

Background:

The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]

UniProt Protein Function:	SMARCE1: Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Required for the coactivation of estrogen responsive promoters by Swi/Snf complexes and the SRC/p160 family of histone acetyltransferases (HATs). Also specifically interacts with the CoREST corepressor resulting in repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Defects in SMARCE1 may be a cause of Coffin-Siris syndrome, a highly variable disease characterized by mental retardation associated with a broad spectrum of different clinical features. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Nuclear receptor co-regulator; Transcription, coactivator/corepressor Chromosomal Location of Human Ortholog: 17q21.2 Cellular Component: nucleoplasm; nuclear chromosome; SWI/SNF complex; protein complex; transcriptional repressor complex; nuclear chromatin; nucleus Molecular Function:ligand-dependent nuclear receptor binding; N-acetyltransferase activity; protein binding; nucleosomal DNA binding; RNA binding; transcription coactivator activity; protein N-terminus binding; chromatin binding Biological Process: chromatin remodeling; regulation of transcription from RNA polymerase II promoter; nervous system development; nucleosome disassembly; metabolic process; ATP-dependent chromatin remodeling; negative regulation of transcription, DNA-dependent Disease: Meningioma, Familial, Susceptibility To
NCBI Summary:	The protein encoded by this gene is part of the large ATP-dependent chromatin remodeling complex SWI/SNF, which is required for transcriptional activation of genes normally repressed by chromatin. The encoded protein, either alone or when in the SWI/SNF complex, can bind to 4-way junction DNA, which is thought to mimic the topology of DNA as it enters or exits the nucleosome. The protein contains a DNA-binding HMG domain, but disruption of this domain does not abolish the DNA-binding or nucleosome-displacement activities of the SWI/SNF complex. Unlike most of the SWI/SNF complex proteins, this protein has no yeast counterpart. [provided by RefSeq, Jul 2008]
UniProt Code:	Q969G3
NCBI GenInfo Identifier:	61247587
NCBI Gene ID:	6605
NCBI Accession:	Q969G3.2
UniProt Secondary Accession:	Q969G3,O43539, B3KMC1, B4DFR4, C0IMW4, C0IMW5, C0IMW7 H7C3F6,
UniProt Related Accession:	Q969G3
Molecular Weight:	411
NCBI Full Name:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
NCBI Synonym Full Names:	SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
NCBI Official Symbol:	SMARCE1
NCBI Official Synonym Symbols:	BAF57
NCBI Protein Information:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1; BRG1-associated factor 57; chromatin remodeling complex BRG1-associated factor 57; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin e1
UniProt Protein Name:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily E member 1
UniProt Synonym Protein Names:	BRG1-associated factor 57; BAF57
Protein Family:	SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily
UniProt Gene Name:	SMARCE1
UniProt Entry Name:	SMCE1_HUMAN