Cell Biology Antibodies 9
Anti-BACE1 Antibody (CAB5266)
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- SKU:
- CAB5266
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-BACE1 Antibody |
| Antibody SKU: | CAB5266 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-322 of human BACE1 (NP_036236.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 22-322 of human BACE1 (NP_036236.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | TQHG IRLP LRSG LGGA PLGL RLPR ETDE EPEE PGRR GSFV EMVD NLRG KSGQ GYYV EMTV GSPP QTLN ILVD TGSS NFAV GAAP HPFL HRYY QRQL SSTY RDLR KGVY VPYT QGKW EGEL GTDL VSIP HGPN VTVR ANIA AITE SDKF FING SNWE GILG LAYA EIAR PDDS LEPF FDSL VKQT HVPN LFSL QLCG AGFP LNQS EVLA SVGG SMII GGID HSLY TGSL WYTP IRRE WYYE VIIV RVEI NGQD LKMD CKEY NYDK SIVD SGTT NLRL PKKV FEAA VKSI KAAS STEK FPDG F |
| Gene ID: | 23621 |
| Uniprot: | P56817 |
| Cellular Location: | Cell surface, Cytoplasmic vesicle membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Single-pass type I membrane protein, trans-Golgi network |
| Calculated MW: | 42kDa/45kDa/48kDa/51kDa/52kDa/55kDa |
| Observed MW: | 75kDa |
| Synonyms: | BACE1, ASP2, BACE, HSPC104 |
| Background: | This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. |
| UniProt Protein Function: | BACE: an integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Cleaves APP at the amino terminus of the A-beta peptide sequence, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated carboxy-terminal fragment which is later release by gamma-secretase. Four splice-variant isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 3.4.23.46; Membrane protein, integral; Protease Chromosomal Location of Human Ortholog: 11q23.2-q23.3 Cellular Component: endoplasmic reticulum lumen; endosome; endosome membrane; Golgi apparatus; integral to plasma membrane; late endosome; multivesicular body; plasma membrane; trans-Golgi network Molecular Function:aspartic-type endopeptidase activity; beta-amyloid binding; beta-aspartyl-peptidase activity; enzyme binding; peptidase activity; protein binding Biological Process: beta-amyloid metabolic process; cellular protein metabolic process; membrane protein ectodomain proteolysis; protein catabolic process; proteolysis |
| NCBI Summary: | This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015] |
| UniProt Code: | P56817 |
| NCBI GenInfo Identifier: | 296434407 |
| NCBI Gene ID: | 23621 |
| NCBI Accession: | P56817.2 |
| UniProt Secondary Accession: | P56817,Q9BYB9, Q9BYC0, Q9BYC1, Q9UJT5, Q9ULS1, A0M8W7 B0YIU9, E9PE65, H7BXJ9, |
| UniProt Related Accession: | P56817 |
| Molecular Weight: | 42,107 Da |
| NCBI Full Name: | Beta-secretase 1 |
| NCBI Synonym Full Names: | beta-secretase 1 |
| NCBI Official Symbol: | BACE1 |
| NCBI Official Synonym Symbols: | ASP2; BACE; HSPC104 |
| NCBI Protein Information: | beta-secretase 1 |
| UniProt Protein Name: | Beta-secretase 1 |
| UniProt Synonym Protein Names: | Aspartyl protease 2; ASP2; Asp 2; Beta-site amyloid precursor protein cleaving enzyme 1; Beta-site APP cleaving enzyme 1; Memapsin-2; Membrane-associated aspartic protease 2 |
| Protein Family: | Beta-secretase |
| UniProt Gene Name: | BACE1 |
| UniProt Entry Name: | BACE1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BACE1 antibody (CAB5266) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunofluorescence analysis of U-251MG cells using BACE1 antibody (CAB5266) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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