Metabolism Antibodies 2

Anti-BAAT Antibody (CAB7646)

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SKU:
CAB7646
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-BAAT Antibody
Antibody SKU:CAB7646
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 309-418 of human BAAT (NP_001692.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Jurkat, BT-474, A-549, HepG2, Mouse liver, Rat liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 309-418 of human BAAT (NP_001692.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YLFP IEEA QGQF LFIV GEGD KTIN SKAH AEQA IGQL KRHG KNNW TLLS YPGA GHLI EPPY SPLC CAST THDL RLHW GGEV IPHA AAQE HAWK EIQR FLRK HLIP DVTS QL
Gene ID:570
Uniprot:Q14032
Cellular Location:Cytoplasm
Calculated MW:46kDa
Observed MW:40-46kDa
Synonyms:BAAT, BACAT, BAT
Background:The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene.
UniProt Protein Function:BAAT: Involved in bile acid metabolism. In liver hepatocytes catalyzes the second step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi. The major components of bile are cholic acid and chenodeoxycholic acid. In a first step the bile acids are converted to an acyl-CoA thioester, either in peroxisomes (primary bile acids deriving from the cholesterol pathway), or cytoplasmic at the endoplasmic reticulum (secondary bile acids). May catalyze the conjugation of primary or secondary bile acids, or both. The conjugation increases the detergent properties of bile acids in the intestine, which facilitates lipid and fat-soluble vitamin absorption. In turn, bile acids are deconjugated by bacteria in the intestine and are recycled back to the liver for reconjugation (secondary bile acids). May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs. Defects in BAAT are involved in familial hypercholanemia (FHCA). FHCA is a disorder characterized by elevated serum bile acid concentrations, itching, and fat malabsorption. Belongs to the C/M/P thioester hydrolase family.
UniProt Protein Details:

Protein type:Hydrolase; Other Amino Acids Metabolism - taurine and hypotaurine; Lipid Metabolism - unsaturated fatty acid biosynthesis; Lipid Metabolism - primary bile acid biosynthesis; EC 2.3.1.65; Transferase; EC 3.1.2.2

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: cytosol; peroxisomal matrix; peroxisome

Molecular Function:glycine N-choloyltransferase activity; N-acyltransferase activity; protein binding; receptor binding; transferase activity, transferring acyl groups

Biological Process: acyl-CoA metabolic process; bile acid biosynthetic process; bile acid metabolic process; glycine metabolic process; taurine metabolic process

Disease: Hypercholanemia, Familial

NCBI Summary:The protein encoded by this gene is a liver enzyme that catalyzes the transfer of C24 bile acids from the acyl-CoA thioester to either glycine or taurine, the second step in the formation of bile acid-amino acid conjugates. The bile acid conjugates then act as a detergent in the gastrointestinal tract, which enhances lipid and fat-soluble vitamin absorption. Defects in this gene are a cause of familial hypercholanemia (FHCA). Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q14032
NCBI GenInfo Identifier:74739811
NCBI Gene ID:570
NCBI Accession:Q14032.1
UniProt Secondary Accession:Q14032,Q3B7W9, Q96L31,
UniProt Related Accession:Q14032
Molecular Weight:46,299 Da
NCBI Full Name:Bile acid-CoA:amino acid N-acyltransferase
NCBI Synonym Full Names:bile acid-CoA:amino acid N-acyltransferase
NCBI Official Symbol:BAAT  
NCBI Official Synonym Symbols:BAT; BACAT  
NCBI Protein Information:bile acid-CoA:amino acid N-acyltransferase
UniProt Protein Name:Bile acid-CoA:amino acid N-acyltransferase
UniProt Synonym Protein Names:Glycine N-choloyltransferase; Long-chain fatty-acyl-CoA hydrolase
Protein Family:Bile acid-CoA:amino acid N-acyltransferase
UniProt Gene Name:BAAT  
UniProt Entry Name:BAAT_HUMAN
Anti-BAAT Antibody (CAB7646)Western blot analysis of extracts of various cell lines, using BAAT antibody (CAB7646) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-BAAT Antibody (CAB7646)Immunohistochemistry of paraffin-embedded human liver using BAAT Rabbit pAb (CAB7646) at dilution of 1:500 (40x lens).
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