Cell Biology Antibodies 1

Anti-B7-H3/CD276 Antibody (CAB10347)

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SKU:
CAB10347
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-B7-H3/CD276 Antibody
Antibody SKU:CAB10347
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human B7-H3/CD276 (NP_001019907.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:U-87MG, 293T, A-549, HeLa
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 100-200 of human B7-H3/CD276 (NP_001019907.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LAQG NASL RLQR VRVA DEGS FTCF VSIR DFGS AAVS LQVA APYS KPSM TLEP NKDL RPGD TVTI TCSS YQGY PEAE VFWQ DGQG VPLT GNVT TSQM ANEQ G
Gene ID:80381
Uniprot:Q5ZPR3
Cellular Location:Membrane, Single-pass type I membrane protein
Calculated MW:33kDa/52kDa/57kDa
Observed MW:115kDa
Synonyms:CD276, 4Ig-B7-H3, B7-H3, B7H3, B7RP-2
Background:The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CD276: May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. Belongs to the immunoglobulin superfamily. BTN/MOG family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 15q23-q24

Cellular Component: external side of plasma membrane; integral to membrane

Molecular Function:protein binding; receptor binding

Biological Process: cell proliferation; immune response; negative regulation of inflammatory response; negative regulation of interferon-gamma biosynthetic process; negative regulation of interleukin-2 biosynthetic process; negative regulation of T cell proliferation; positive regulation of bone mineralization; positive regulation of interferon-gamma biosynthetic process; positive regulation of osteoblast differentiation; positive regulation of T cell proliferation; regulation of immune response; T cell activation

NCBI Summary:The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
UniProt Code:Q5ZPR3
NCBI GenInfo Identifier:74757248
NCBI Gene ID:80381
NCBI Accession:Q5ZPR3.1
UniProt Secondary Accession:Q5ZPR3,Q6P5Y4, Q6UXI2, Q8NBI8, Q8NC34, Q8NCB6, Q9BXR1
UniProt Related Accession:Q5ZPR3
Molecular Weight:
NCBI Full Name:CD276 antigen
NCBI Synonym Full Names:CD276 molecule
NCBI Official Symbol:CD276  
NCBI Official Synonym Symbols:B7H3; B7-H3; B7RP-2; 4Ig-B7-H3  
NCBI Protein Information:CD276 antigen
UniProt Protein Name:CD276 antigen
UniProt Synonym Protein Names:4Ig-B7-H3; B7 homolog 3; B7-H3; Costimulatory molecule; CD_antigen: CD276
Protein Family:CD276 antigen
UniProt Gene Name:CD276  
UniProt Entry Name:CD276_HUMAN
Anti-B7-H3/CD276 Antibody (CAB10347)Western blot analysis of extracts of various cell lines, using B7-H3/CD276 antibody (CAB10347) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
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