Cell Biology Antibodies 5

Anti-B4GALT4 Antibody (CAB14693)

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SKU:
CAB14693
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-B4GALT4 Antibody
Antibody SKU:CAB14693
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-344 of human B4GALT4 (NP_003769.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:293T, HT-29, Mouse kidney, Mouse thymus, Mouse lung, Rat kidney, Rat thymus, Rat lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 35-344 of human B4GALT4 (NP_003769.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VGAI QEIP KAKE FMAN FHKT LILG KGKT LTNE ASTK KVEL DNCP SVSP YLRG QSKL IFKP DLTL EEVQ AENP KVSR GRYR PQEC KALQ RVAI LVPH RNRE KHLM YLLE HLHP FLQR QQLD YGIY VIHQ AEGK KFNR AKLL NVGY LEAL KEEN WDCF IFHD VDLV PEND FNLY KCEE HPKH LVVG RNST GYRL RYSG YFGG VTAL SREQ FFKV NGFS NNYW GWGG EDDD LRLR VELQ RMKI SRPL PEVG KYTM VFHT RDKG NEVN AERM KLLH QVSR VWRT DGLS SCSY KLVS VEHN PLYI NITV DFWF GA
Gene ID:8702
Uniprot:O60513
Cellular Location:Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein
Calculated MW:40kDa
Observed MW:40kDa
Synonyms:B4GALT4, B4Gal-T4, beta4Gal-T4, beta-1
Background:This gene is one of seven beta-1, 4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1, 4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene.
UniProt Code:O60513
NCBI GenInfo Identifier:13123971
NCBI Gene ID:8702
NCBI Accession:O60513.1
UniProt Secondary Accession:O60513,Q68D68, Q9BSW3, Q9C078,
UniProt Related Accession:O60513
Molecular Weight:344
NCBI Full Name:Beta-1,4-galactosyltransferase 4
NCBI Synonym Full Names:UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4
NCBI Official Symbol:B4GALT4  
NCBI Official Synonym Symbols:B4Gal-T4; beta4Gal-T4  
NCBI Protein Information:beta-1,4-galactosyltransferase 4; beta-1,4-GalTase 4; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
UniProt Protein Name:Beta-1,4-galactosyltransferase 4
UniProt Synonym Protein Names:UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4Including the following 2 domains:N-acetyllactosamine synthase (EC:2.4.1.90)Alternative name(s):Nal synthase
Protein Family:Beta-1,4-galactosyltransferase
UniProt Gene Name:B4GALT4  
UniProt Entry Name:B4GT4_HUMAN
Anti-B4GALT4 Antibody (CAB14693)Western blot analysis of extracts of various cell lines, using B4GALT4 antibody (CAB14693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-B4GALT4 Antibody (CAB14693)Immunofluorescence analysis of HeLa cells using B4GALT4 antibody (CAB14693) at dilution of 1:100. Blue: DAPI for nuclear staining.
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system_update_altDatasheetHuman B4GALT4 Recombinant Protein β-1,4-galactosyltransferase 4 (B4GALT4) is a single-pass type II membrane protein that belongs to the Glycosyltransferase 7 family