Metabolism Antibodies 2

Anti-B4GALNT1 Antibody (CAB3874)

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SKU:
CAB3874
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-B4GALNT1 Antibody
Antibody SKU:CAB3874
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-240 of human B4GALNT1 (NP_001263398.1).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100
Reactivity:Human
Positive Samples:U-87MG, A375, MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 26-240 of human B4GALNT1 (NP_001263398.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:STRD APGL RLPL APWA PPQS PRRP ELPD LAPE PRYA HIPV RIKE QVVG LLAW NNCS CESS GGGL PLPF QKQV RAID LTKA FDPA ELRA ASAT REQE FQAF LSRS QSPA DQLL IAPA NSPL QYPL QGVE VQPL RSIL VPGL SLQA ASGQ EVYQ VNLT ASLG TWDV AGEV TGVT LTGE GQAD LTLV SPGL DQLN RQLQ LVTY SSRS YQTN TADT GAR
Gene ID:2583
Uniprot:Q00973
Cellular Location:Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:35kDa/52kDa/58kDa
Observed MW:80kDa
Synonyms:B4GALNT1, GALGT, GALNACT, GalNAc-T, SPG26, beta-1
Background:GM2 and GD2 gangliosides are sialic acid-containing glycosphingolipids. GalNAc-T is the enzyme involved in the biosynthesis of G(M2) and G(D2) glycosphingolipids. GalNAc-T catalyzes the transfer of GalNAc into G(M3) and G(D3) by a beta-1, 4 linkage, resulting in the synthesis of G(M2) and G(D2), respectively. Three transcript variants encoding different isoforms have been found for this gene.
UniProt Code:Q00973
NCBI GenInfo Identifier:1168736
NCBI Gene ID:2583
NCBI Accession:Q00973.2
UniProt Secondary Accession:Q00973,Q8N636, B4DE26,
UniProt Related Accession:Q00973
Molecular Weight:533
NCBI Full Name:Beta-1,4 N-acetylgalactosaminyltransferase 1
NCBI Synonym Full Names:beta-1,4-N-acetyl-galactosaminyl transferase 1
NCBI Official Symbol:B4GALNT1  
NCBI Official Synonym Symbols:GALGT; SPG26; GALNACT; GalNAc-T  
NCBI Protein Information:beta-1,4 N-acetylgalactosaminyltransferase 1; beta1,4GalNAc-T; GM2/GD2 synthase; GD2 synthase, GM2 synthase; UDP-Gal:betaGlcNAc beta-1,4-N-acetylgalactosaminyltransferase transferase 1; UDP-N-acetyl-alpha-D-galactosamine:(N-acetylneuraminyl)-galactosylglucosylceramide N-acetylgalactosaminyltransferase (GalNAc-T)
UniProt Protein Name:Beta-1,4 N-acetylgalactosaminyltransferase 1
UniProt Synonym Protein Names:(N-acetylneuraminyl)-galactosylglucosylceramide; GM2/GD2 synthase; GalNAc-T
Protein Family:Beta-1,4 N-acetylgalactosaminyltransferase
UniProt Gene Name:B4GALNT1  
UniProt Entry Name:B4GN1_HUMAN
Anti-B4GALNT1 Antibody (CAB3874)Western blot analysis of extracts of various cell lines, using B4GALNT1 antibody (CAB3874) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
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