Name: Anti-B3GNT5 Antibody (CAB14428)
Brand: Cell Biology Antibodies 5
SKU: CAB14428
Availability: OutOfStock

Antibody Name:	Anti-B3GNT5 Antibody
Antibody SKU:	CAB14428
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 36-378 of human B3GNT5 (NP_114436.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	HT-29, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 36-378 of human B3GNT5 (NP_114436.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DNHI VSHM KSYS YRYL INSY DFVN DTLS LKHT SAGP RYQY LINH KEKC QAQD VLLL LFVK TAPE NYDR RSGI RRTW GNEN YVRS QLNA NIKT LFAL GTPN PLEG EELQ RKLA WEDQ RYND IIQQ DFVD SFYN LTLK LLMQ FSWA NTYC PHAK FLMT ADDD IFIH MPNL IEYL QSLE QIGV QDFW IGRV HRGA PPIR DKSS KYYV SYEM YQWP AYPD YTAG AAYV ISGD VAAK VYEA SQTL NSSL YIDD VFMG LCAN KIGI VPQD HVFF SGEG KTPY HPCI YEKM MTSH GHLE DLQD LWKN ATDP KVKT ISKG FFGQ IYCR LMKI ILLC KISY VDTY PCRA AFI
Gene ID:	84002
Uniprot:	Q9BYG0
Cellular Location:	Golgi apparatus membrane, Single-pass type II membrane protein
Calculated MW:	44kDa
Observed MW:	44kDa

Synonyms:	B3GNT5, B3GN-T5
Background:	This gene encodes a member of the beta-1, 3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids.

UniProt Protein Function:	B3GNT5: Beta-1,3-N-acetylglucosaminyltransferase that plays a key role in the synthesis of lacto- or neolacto-series carbohydrate chains on glycolipids, notably by participating in biosynthesis of HNK-1 and Lewis X carbohydrate structures. Has strong activity toward lactosylceramide (LacCer) and neolactotetraosylceramide (nLc(4)Cer; paragloboside), resulting in the synthesis of Lc(3)Cer and neolactopentaosylceramide (nLc(5)Cer), respectively. Probably plays a central role in regulating neolacto-series glycolipid synthesis during embryonic development. Belongs to the glycosyltransferase 31 family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Transferase; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; EC 2.4.1.206 Chromosomal Location of Human Ortholog: 3q28 Cellular Component: Golgi membrane; integral to membrane; intracellular Molecular Function:lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase activity; galactosyltransferase activity; beta-galactosyl-N-acetylglucosaminylgalactosylglucosyl-ceramide beta-1,3-acetylglucosaminyltransferase activity Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; central nervous system development; O-glycan processing; glycolipid biosynthetic process; protein amino acid glycosylation; post-translational protein modification
NCBI Summary:	This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II membrane protein. It exhibits strong activity to transfer GlcNAc to glycolipid substrates and is identified as the most likely candidate for lactotriaosylceramide synthase. This enzyme is essential for the expression of Lewis X epitopes on glycolipids. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9BYG0
NCBI GenInfo Identifier:	74733473
NCBI Gene ID:	84002
NCBI Accession:	Q9BYG0.1
UniProt Secondary Accession:	Q9BYG0,Q59FE3, Q7L9Z5, Q8WWP9, D3DNS5,
UniProt Related Accession:	Q9BYG0
Molecular Weight:	378
NCBI Full Name:	Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
NCBI Synonym Full Names:	UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 5
NCBI Official Symbol:	B3GNT5
NCBI Official Synonym Symbols:	B3GN-T5; beta3Gn-T5
NCBI Protein Information:	lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase; BGnT-5; lc3 synthase; beta-1,3-Gn-T5; lc(3)Cer synthase; lactotriaosylceramide synthase; beta-1,3-N-acetylglucosaminyltransferase 5; beta-1,3-N-acetylglucosaminyltransferase bGnT-5; beta 1,3 N-acetyglucosaminyltransferase Lc3 synthase; UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5
UniProt Protein Name:	Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
UniProt Synonym Protein Names:	Lactotriaosylceramide synthase; Lc(3)Cer synthase; Lc3 synthase; UDP-GlcNAc:beta-Gal beta-1,3-N-acetylglucosaminyltransferase 5; BGnT-5; Beta-1,3-Gn-T5; Beta-1,3-N-acetylglucosaminyltransferase 5; Beta3Gn-T5
Protein Family:	Lactosylceramide 1,3-N-acetyl-beta-D-glucosaminyltransferase
UniProt Gene Name:	B3GNT5
UniProt Entry Name:	B3GN5_HUMAN

Western blot analysis of extracts of various cell lines, using B3GNT5 antibody (CAB14428) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

B3GNT5 Antibody (PACO07965)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)