Cell Biology Antibodies 9

Anti-AZU1 Antibody (CAB6132)

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SKU:
CAB6132
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-AZU1 Antibody
Antibody SKU:CAB6132
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 27-251 of human AZU1 (NP_001691.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 27-251 of human AZU1 (NP_001691.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:IVGG RKAR PRQF PFLA SIQN QGRH FCGG ALIH ARFV MTAA SCFQ SQNP GVST VVLG AYDL RRRE RQSR QTFS ISSM SENG YDPQ QNLN DLML LQLD REAN LTSS VTIL PLPL QNAT VEAG TRCQ VAGW GSQR SGGR LSRF PRFV NVTV TPED QCRP NNVC TGVL TRRG GICN GDGG TPLV CEGL AHGV ASFS LGPC GRGP DFFT RVAL FRDW IDGV LNNP GPGP A
Gene ID:566
Uniprot:P20160
Cellular Location:Cytoplasmic granule
Calculated MW:26kDa
Observed MW:35kDa
Synonyms:AZU1, AZAMP, AZU, CAP37, HBP, HUMAZUR, NAZC, hHBP
Background:Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. This gene encodes a preproprotein that is proteolytically processed to generate a mature azurophil granule antibiotic protein, with monocyte chemotactic and antimicrobial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation.
UniProt Protein Function:AZU1: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram- negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. Belongs to the peptidase S1 family. Elastase subfamily.
UniProt Protein Details:

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: azurophil granule; extracellular region

Molecular Function:heparin binding; toxin binding; serine-type endopeptidase activity

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; positive regulation of cell adhesion; microglial cell activation; glial cell migration; proteolysis; defense response to Gram-negative bacterium; macrophage chemotaxis; induction of positive chemotaxis; cellular extravasation; monocyte activation; positive regulation of phagocytosis; positive regulation of MHC class II biosynthetic process; positive regulation of fractalkine biosynthetic process; protein kinase C activation; positive regulation of interleukin-1 beta biosynthetic process; protein processing; immune response; regulation of vascular permeability; inflammatory response; negative regulation of apoptosis

NCBI Summary:Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antimicrobial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Dec 2014]
UniProt Code:P20160
NCBI GenInfo Identifier:416746
NCBI Gene ID:566
NCBI Accession:P20160.3
UniProt Secondary Accession:P20160,P80014, Q52LG4, Q9UCM1, Q9UCT5,
UniProt Related Accession:P20160
Molecular Weight:251
NCBI Full Name:Azurocidin
NCBI Synonym Full Names:azurocidin 1
NCBI Official Symbol:AZU1  
NCBI Official Synonym Symbols:AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR  
NCBI Protein Information:azurocidin; neutrophil azurocidin; heparin-binding protein; cationic antimicrobial protein 37; cationic antimicrobial protein CAP37
UniProt Protein Name:Azurocidin
UniProt Synonym Protein Names:Cationic antimicrobial protein CAP37; Heparin-binding protein; HBP
Protein Family:Azurocidin
UniProt Gene Name:AZU1  
UniProt Entry Name:CAP7_HUMAN
Anti-AZU1 Antibody (CAB6132)Western blot analysis of extracts of LO2 cells, using AZU1 Antibody (CAB6132) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
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