Metabolism Antibodies 3

Anti-AZIN2 Antibody (CAB15936)

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SKU:
CAB15936
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-AZIN2 Antibody
Antibody SKU:CAB15936
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human AZIN2 (NP_443724.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:U-87MG, Mouse pancreas, Mouse kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-360 of human AZIN2 (NP_443724.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MAGY LSES DFVM VEEG FSTR DLLK ELTL GASQ ATTD EVAA FFVA DLGA IVRK HFCF LKCL PRVR PFYA VKCN SSPG VLKV LAQL GLGF SCAN KAEM ELVQ HIGI PASK IICA NPCK QIAQ IKYA AKHG IQLL SFDN EMEL AKVV KSHP SAKM VLCI ATDD SHSL SCLS LKFG VSLK SCRH LLEN AKKH HVEV VGVS FHIG SGCP DPQA YAQS IADA RLVF EMGT ELGH KMHV LDLG GGFP GTEG AKVR FEEI ASVI NSAL DLYF PEGC GVDI FAEL GRYY VTSA FTVA VSII AKKE VLLD QPGR EEEN GSTS KTIV YHLD EGVY GIFN SVLF DNIC PTPI LQKK PSTE QPLY SSSL WGPA
Gene ID:113451
Uniprot:Q96A70
Cellular Location:Cell projection, Cytoplasm, Cytoplasmic granule, Cytoplasmic vesicle, Endoplasmic reticulum-Golgi intermediate compartment, Golgi apparatus, Membrane, Nucleus, Perikaryon, axon, cis-Golgi network, dendrite, perinuclear region, trans-Golgi network
Calculated MW:22kDa/39kDa/40kDa/49kDa/51kDa
Observed MW:50kDa
Synonyms:AZIN2, ADC, AZI2, AZIB1, ODC-p, ODC1L, ODCp
Background:The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of antizyme inhibitor 2 has also been observed in brains of patients with Alzheimer's disease. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). Alternatively spliced transcript variants have been described for this gene.
UniProt Protein Function:ADC: Decarboxylates L-arginine to agmatine. Truncated splice isoforms probably lack activity. Belongs to the Orn/Lys/Arg decarboxylase class-II family. 5 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Amino Acid Metabolism - arginine and proline; EC 4.1.1.19; Lyase; Mitochondrial

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: axon; cis-Golgi network; cytoplasmic vesicle; cytosol; dendrite; ER-Golgi intermediate compartment membrane; nucleus; perikaryon; perinuclear region of cytoplasm; trans-Golgi network; transport vesicle

Molecular Function:arginine decarboxylase activity; ornithine decarboxylase activator activity; protein binding; putrescine transmembrane transporter activity

Biological Process: negative regulation of protein catabolic process; positive regulation of catalytic activity; putrescine biosynthetic process from ornithine; putrescine transport

NCBI Summary:The protein encoded by this gene belongs to the antizyme inhibitor family, which plays a role in cell growth and proliferation by maintaining polyamine homeostasis within the cell. Antizyme inhibitors are homologs of ornithine decarboxylase (ODC, the key enzyme in polyamine biosynthesis) that have lost the ability to decarboxylase ornithine; however, retain the ability to bind to antizymes. Antizymes negatively regulate intracellular polyamine levels by binding to ODC and targeting it for degradation, as well as by inhibiting polyamine uptake. Antizyme inhibitors function as positive regulators of polyamine levels by sequestering antizymes and neutralizing their effect. This gene encodes antizyme inhibitor 2, the second member of this gene family. Like antizyme inhibitor 1, antizyme inhibitor 2 interacts with all 3 antizymes and stimulates ODC activity and polyamine uptake. However, unlike antizyme inhibitor 1, which is ubiquitously expressed and localized in the nucleus and cytoplasm, antizyme inhibitor 2 is predominantly expressed in the brain and testis and localized in the endoplasmic reticulum-golgi intermediate compartment. Recent studies indicate that antizyme inhibitor 2 is also expressed in specific cell types in ovaries, adrenal glands and pancreas, and in mast cells. The exact function of this gene is not known, however, available data suggest its role in cell growth, spermiogenesis, vesicular trafficking and secretion. Accumulation of antizyme inhibitor 2 has also been observed in brains of patients with Alzheimer's disease. There has been confusion in literature and databases over the nomenclature of this gene, stemming from an earlier report that a human cDNA clone (identical to ODCp/AZIN2) had arginine decarboxylase (ADC) activity (PMID:14738999). Subsequent studies in human and mouse showed that antizyme inhibitor 2 was devoid of arginine decarboxylase activity (PMID:19956990). Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Sep 2014]
UniProt Code:Q96A70
NCBI GenInfo Identifier:649572333
NCBI Gene ID:113451
NCBI Accession:NP_001280491.1
UniProt Secondary Accession:Q96A70,Q5TIF4, Q5TIF5, Q5TIF6, Q8TF56, Q96L54, Q96L55 Q96L56, Q96L57, Q96MD9, B2RDU5, D3DPQ9,
UniProt Related Accession:Q96A70
Molecular Weight:22,300 Da
NCBI Full Name:antizyme inhibitor 2 isoform 1
NCBI Synonym Full Names:antizyme inhibitor 2
NCBI Official Symbol:AZIN2  
NCBI Official Synonym Symbols:ADC; AZI2; ODCp; AZIB1; ODC-p; ODC1L  
NCBI Protein Information:antizyme inhibitor 2
UniProt Protein Name:Antizyme inhibitor 2
UniProt Synonym Protein Names:Arginine decarboxylase; ADC; ARGDC; Ornithine decarboxylase-like protein; ODC-like protein; ornithine decarboxylase paralog; ODC-p
Protein Family:Antizyme inhibitor
UniProt Gene Name:AZIN2  
Anti-AZIN2 Antibody (CAB15936)Western blot analysis of extracts of various cell lines, using AZIN2 antibody (CAB15936) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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