Cell Cycle Antibodies 2

Anti-AURKC Antibody (CAB16977)

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SKU:
CAB16977
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-AURKC Antibody (CAB16977)
Antibody SKU:CAB16977
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AURKC (NP_001015878.1).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:U-251MG
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human AURKC (NP_001015878.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSSP RAVV QLGK AQPA GEEL ATAN QTAQ QPSS PAMR RLTV DDFE IGRP LGKG KFGN VYLA RLKE SHFI VALK VLFK SQIE KEGL EHQL RREI EIQA HLQH
Cellular Location:Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, spindle
Calculated MW:32kDa/33kDa/35kDa
Observed MW:36KDa
Synonyms:AURKC, AIE2, AIK3, ARK3, AurC, HEL-S-90, SPGF5, STK13, aurora-C
Background:This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants.
Western blot analysis of extracts of U-251MG cells, using AURKC antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.Western blot analysis of extracts of U-251MG cells, using AURKC antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
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