Cell Biology Antibodies 16
Anti-AurkB Antibody (CAB1020)
- SKU:
- CAB1020
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
Antibody Name: | Anti-AurkB Antibody |
Antibody SKU: | CAB1020 |
Antibody Size: | 20uL, 50uL, 100uL |
Application: | WB IHC |
Reactivity: | Human, Mouse |
Host Species: | Rabbit |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-344 of human AurkB (NP_004208.2). |
Application: | WB IHC |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse |
Positive Samples: | HeLa, NIH/3T3, MCF7, SW480, Mouse brain, Mouse craniofacial, Mouse liver, Mouse stomach, Mouse intestine |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-344 of human AurkB (NP_004208.2). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | MAQK ENSY PWPY GRQT APSG LSTL PQRV LRKE PVTP SALV LMSR SNVQ PTAA PGQK VMEN SSGT PDIL TRHF TIDD FEIG RPLG KGKF GNVY LARE KKSH FIVA LKVL FKSQ IEKE GVEH QLRR EIEI QAHL HHPN ILRL YNYF YDRR RIYL ILEY APRG ELYK ELQK SCTF DEQR TATI MEEL ADAL MYCH GKKV IHRD IKPE NLLL GLKG ELKI ADFG WSVH APSL RRKT MCGT LDYL PPEM IEGR MHNE KVDL WCIG VLCY ELLV GNPP FESA SHNE TYRR IVKV DLKF PASV PMGA QDLI SKLL RHNP SERL PLAQ VSAH PWVR ANSR RVLP PSAL QSVA |
Gene ID: | 9212 |
Uniprot: | Q96GD4 |
Cellular Location: | Chromosome, Cytoplasm, Midbody, Nucleus, centromere, cytoskeleton, spindle |
Calculated MW: | 16kDa/34kDa/35kDa/39kDa |
Observed MW: | 39kDa |
Synonyms: | AIK2, AIM-1, AIM1, ARK2, AurB, IPL1, PPP1R48, STK12, STK5, aurkb-sv1, aurkb-sv2, AURKB, Aurora B |
Background: | This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. |
UniProt Protein Function: | AurB: a member of the AUR family of kinases. May be directly involved in regulating the cleavage of polar spindle microtubules and is a key regulator for the onset of cytokinesis during mitosis. Expressed during S and G2/M phase and expression is upregulated in cancer cells during M phase. Localized to the midzone of central spindle in late anaphase and concentrated into the midbody in telophase and cytokinesis. Colocalizes with gamma tubulin in the mid-body. Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Overexpressed in colorectal and other cancer cell lines and thought to cause aneuploidy via histone phosphorylation. |
UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, Other; Other group; AUR family Chromosomal Location of Human Ortholog: 17p13.1 Cellular Component: chromocenter; condensed nuclear chromosome, pericentric region; cytosol; intercellular bridge; kinetochore; midbody; nucleoplasm; nucleus; spindle; spindle microtubule; spindle midzone; spindle pole centrosome Molecular Function:ATP binding; histone serine kinase activity; metal ion binding; protein binding; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity Biological Process: abscission; aging; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; attachment of spindle microtubules to kinetochore; cell proliferation; cellular protein metabolic process; histone modification; mitotic cell cycle; negative regulation of B cell apoptosis; negative regulation of cytokinesis; negative regulation of protein binding; negative regulation of transcription from RNA polymerase II promoter; positive regulation of cytokinesis; positive regulation of telomerase activity; positive regulation of telomere maintenance via telomerase; post-translational protein modification; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein sumoylation; regulation of chromosome segregation; small GTPase mediated signal transduction; spindle checkpoint; spindle midzone assembly involved in mitosis; spindle stabilization |
NCBI Summary: | This gene encodes a member of the aurora kinase subfamily of serine/threonine kinases. The genes encoding the other two members of this subfamily are located on chromosomes 19 and 20. These kinases participate in the regulation of alignment and segregation of chromosomes during mitosis and meiosis through association with microtubules. A pseudogene of this gene is located on chromosome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2015] |
UniProt Code: | Q96GD4 |
NCBI GenInfo Identifier: | 317373473 |
NCBI Gene ID: | 9212 |
NCBI Accession: | Q96GD4.3 |
UniProt Secondary Accession: | Q96GD4,O14630, O60446, O95083, Q96DV5, Q9UQ46, B4DNM4 C7G533, C7G534, C7G535, D3DTR4, J9JID1, |
UniProt Related Accession: | Q96GD4 |
Molecular Weight: | 39,467 Da |
NCBI Full Name: | Aurora kinase B |
NCBI Synonym Full Names: | aurora kinase B |
NCBI Official Symbol: | AURKBÂ Â |
NCBI Official Synonym Symbols: | AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2Â Â |
NCBI Protein Information: | aurora kinase B |
UniProt Protein Name: | Aurora kinase B |
UniProt Synonym Protein Names: | Aurora 1; Aurora- and IPL1-like midbody-associated protein 1; AIM-1; Aurora/IPL1-related kinase 2; ARK-2; Aurora-related kinase 2; STK-1; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B |
Protein Family: | Aurora kinase |
UniProt Gene Name: | AURKBÂ Â |
UniProt Entry Name: | AURKB_HUMAN |