Metabolism Antibodies 3

Anti-ATPAF2 Antibody (CAB15928)

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SKU:
CAB15928
Product Type:
Antibody
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-ATPAF2 Antibody
Antibody SKU:CAB15928
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 41-200 of human ATPAF2 (NP_663729.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Mouse
Positive Samples:Mouse heart, Mouse kidney, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 41-200 of human ATPAF2 (NP_663729.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:APPT ERKR FYQN VSIT QGEG GFEI NLDH RKLK TPQA KLFT VPSE ALAI AVAT EWDS QQDT IKYY TMHL TTLC NTSL DNPT QRNK DQLI RAAV KFLD TDTI CYRV EEPE TLVE LQRN EWDP IIEW AEKR YGVE ISSS TSIM GPSI PAKT REVL VSHL ASYN
Gene ID:91647
Uniprot:Q8N5M1
Cellular Location:Mitochondrion
Calculated MW:32kDa
Observed MW:38kDa
Synonyms:ATPAF2, ATP12, ATP12p, LP3663, MC5DN1
Background:This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. This gene is located within the Smith-Magenis syndrome region on chromosome 17. An alternatively spliced transcript variant has been described, but its biological validity has not been determined.
UniProt Protein Function:ATPAF2: May play a role in the assembly of the F1 component of the mitochondrial ATP synthase (ATPase). Defects in ATPAF2 are a cause of mitochondrial complex V deficiency nuclear type 1 (MC5DN1). A mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid. Belongs to the ATP12 family.
UniProt Protein Details:

Protein type:Mitochondrial

Chromosomal Location of Human Ortholog: 17p11.2

Cellular Component: cytosol; nuclear speck

Molecular Function:protein binding

Disease: Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 1

NCBI Summary:This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. This gene is located within the Smith-Magenis syndrome region on chromosome 17. An alternatively spliced transcript variant has been described, but its biological validity has not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:Q8N5M1
NCBI GenInfo Identifier:21735485
NCBI Gene ID:91647
NCBI Accession:NP_663729.1
UniProt Secondary Accession:Q8N5M1,Q6XYC7, A6NDE5, A8K2J2,
UniProt Related Accession:Q8N5M1
Molecular Weight:32.6kD
NCBI Full Name:ATP synthase mitochondrial F1 complex assembly factor 2
NCBI Synonym Full Names:ATP synthase mitochondrial F1 complex assembly factor 2
NCBI Official Symbol:ATPAF2  
NCBI Official Synonym Symbols:ATP12; ATP12p; LP3663; MC5DN1  
NCBI Protein Information:ATP synthase mitochondrial F1 complex assembly factor 2
UniProt Protein Name:ATP synthase mitochondrial F1 complex assembly factor 2
UniProt Synonym Protein Names:ATP12 homolog
Protein Family:ATP synthase
UniProt Gene Name:ATPAF2  
Anti-ATPAF2 Antibody (CAB15928)Western blot analysis of extracts of various cell lines, using ATPAF2 antibody (CAB15928) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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