Name: Anti-ATP8A1 Antibody (CAB3369)
Brand: Cell Biology Antibodies 8
SKU: CAB3369
Availability: OutOfStock

Antibody Name:	Anti-ATP8A1 Antibody
Antibody SKU:	CAB3369
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 420-545 of human ATP8A1 (NP_001098999.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 420-545 of human ATP8A1 (NP_001098999.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QFKK CTIA GVAY GQNS QFGD EKTF SDSS LLEN LQNN HPTA PIIC EFLT MMAV CHTA VPER EGDK IIYQ AASP DEGA LVRA AKQL NFVF TGRT PDSV IIDS LGQE ERYE LLNV LEFT SARK RMSV IV
Gene ID:	10396
Uniprot:	Q9Y2Q0
Cellular Location:	Cell membrane, Cytoplasmic granule, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Multi-pass membrane protein, chromaffin granule membrane, secretory vesicle
Calculated MW:	129kDa/131kDa
Observed MW:	170kDa

Synonyms:

ATP8A1, ATPASEII, ATPIA, ATPP2

Background:

The P-type adenosinetriphosphatases (P-type ATPases) are a family of proteins which use the free energy of ATP hydrolysis to drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily catalyzes transport of heavy metal ions. Another subfamily transports non-heavy metal ions (NMHI). The protein encoded by this gene is a member of the third subfamily of P-type ATPases and acts to transport amphipaths, such as phosphatidylserine. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	ATP8A1: May play a role in the transport of aminophospholipids from the outer to the inner leaflet of various membranes and the maintenance of asymmetric distribution of phospholipids, mainly in secretory vesicles. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IV subfamily. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Transporter; EC 3.6.3.1; Hydrolase; Membrane protein, multi-pass; Membrane protein, integral; Transporter, ion channel Chromosomal Location of Human Ortholog: 4p13 Cellular Component: Golgi apparatus; membrane; endoplasmic reticulum; plasma membrane; integral to membrane Molecular Function:phospholipid-translocating ATPase activity; protein binding; magnesium ion binding; ATP binding; cation-transporting ATPase activity Biological Process: phospholipid translocation; metabolic process; learning; transmembrane transport; cation transport; positive regulation of cell migration
NCBI Summary:	The P-type adenosinetriphosphatases (P-type ATPases) are a family of proteins which use the free energy of ATP hydrolysis to drive uphill transport of ions across membranes. Several subfamilies of P-type ATPases have been identified. One subfamily catalyzes transport of heavy metal ions. Another subfamily transports non-heavy metal ions (NMHI). The protein encoded by this gene is a member of the third subfamily of P-type ATPases and acts to transport amphipaths, such as phosphatidylserine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9Y2Q0
NCBI GenInfo Identifier:	8134331
NCBI Gene ID:	10396
NCBI Accession:	Q9Y2Q0.1
UniProt Secondary Accession:	Q9Y2Q0,Q32M35, Q32M36, Q4W5J7, Q4W5P2,
UniProt Related Accession:	Q9Y2Q0
Molecular Weight:	1164
NCBI Full Name:	Phospholipid-transporting ATPase IA
NCBI Synonym Full Names:	ATPase, aminophospholipid transporter (APLT), class I, type 8A, member 1
NCBI Official Symbol:	ATP8A1
NCBI Official Synonym Symbols:	ATPIA; ATPP2; ATPASEII
NCBI Protein Information:	phospholipid-transporting ATPase IA; ATPase II; chromaffin granule ATPase II; aminophospholipid translocase; ATPase class I type 8A member 1; probable phospholipid-transporting ATPase IA; P4-ATPase flippase complex alpha subunit ATP8A1
UniProt Protein Name:	Phospholipid-transporting ATPase IA
UniProt Synonym Protein Names:	ATPase class I type 8A member 1; Chromaffin granule ATPase II; P4-ATPase flippase complex alpha subunit ATP8A1
Protein Family:	Phospholipid-transporting ATPase
UniProt Gene Name:	ATP8A1
UniProt Entry Name:	AT8A1_HUMAN

Western blot analysis of extracts of various cell lines, using ATP8A1 antibody (CAB3369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

ATP8A1 Antibody (PACO07944)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)