Antibody Name:	Anti-ATP7B Antibody
Antibody SKU:	CAB5676
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1055-1354 of human ATP7B (NP_001230111.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	BT-474, HL-60, Mouse liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1055-1354 of human ATP7B (NP_001230111.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	SDAM TDHE MKGQ TAIL VAID GVLC GMIA IADA VKQE AALA VHTL QSMG VDVV LITG DNRK TARA IATQ VGIN KVFA EVLP SHKV AKVQ ELQN KGKK VAMV GDGV NDSP ALAQ ADMG VAIG TGTD VAIE AADV VLIR NDLL DVVA SIHL SKRT VRRI RINL VLAL IYNL VGIP IAAG VFMP IGIV LQPW MGSA AMAA SSVS VVLS SLQL KCYK KPDL ERYE AQAH GHMK PLTA SQVS VHIG MDDR WRDS PRAT PWDQ VSYV SQVS LSSL TSDK PSRH SAAA DDDG DKWS LLLN GRDE EQYI
Gene ID:	540
Uniprot:	P35670
Cellular Location:	Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Mitochondrion, Multi-pass membrane protein, Multi-pass membrane protein, trans-Golgi network membrane
Calculated MW:	133kDa/145kDa/155kDa/157kDa
Observed MW:	150kDa

Synonyms:

ATP7B, PWD, WC1, WD, WND

Background:

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD).

UniProt Protein Function:	ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Transporter, ion channel; Membrane protein, multi-pass; EC 3.6.3.54; Vesicle; Transporter; Hydrolase Chromosomal Location of Human Ortholog: 13q14.3 Cellular Component: basolateral plasma membrane; cytoplasmic membrane-bound vesicle; Golgi membrane; integral to plasma membrane; late endosome; membrane; mitochondrion; perinuclear region of cytoplasm; tight junction; trans-Golgi network Molecular Function:ATP binding; copper ion binding; copper-exporting ATPase activity; protein binding; zinc ion binding Biological Process: cellular copper ion homeostasis; cellular zinc ion homeostasis; circadian rhythm; copper ion export; copper ion import; copper ion transport; intracellular copper ion transport; lactation; metabolic process; response to cAMP; response to copper ion; response to zinc ion; sequestering of calcium ion; transmembrane transport Disease: Wilson Disease
NCBI Summary:	This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008]
UniProt Code:	P35670
NCBI GenInfo Identifier:	239938919
NCBI Gene ID:	540
NCBI Accession:	P35670.4
UniProt Secondary Accession:	P35670,Q16318, Q16319, Q4U3V3, Q59FJ9, Q5T7X7,
UniProt Related Accession:	P35670
Molecular Weight:	155,125 Da
NCBI Full Name:	Copper-transporting ATPase 2
NCBI Synonym Full Names:	ATPase copper transporting beta
NCBI Official Symbol:	ATP7B
NCBI Official Synonym Symbols:	WD; PWD; WC1; WND
NCBI Protein Information:	copper-transporting ATPase 2
UniProt Protein Name:	Copper-transporting ATPase 2
UniProt Synonym Protein Names:	Copper pump 2; Wilson disease-associated protein
UniProt Gene Name:	ATP7B
UniProt Entry Name:	ATP7B_HUMAN

	Western blot analysis of extracts of various cell lines, using ATP7B antibody (CAB5676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using ATP7B antibody (CAB5676). Blue: DAPI for nuclear staining.