Anti-ATP6V1H Antibody (CAB10604)

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SKU:
CAB10604
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG

Description

Antibody Name:Anti-ATP6V1H Antibody (CAB10604)
Antibody SKU:CAB10604
Antibody Size:50µL, 100µL
Application:Western blotting
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 254-483 of human ATP6V1H (NP_057025.2).
Application:Western blotting
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:LO2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 254-483 of human ATP6V1H (NP_057025.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PQMC EHLR RYNI IPVL SDIL QESV KEKV TRII LAAF RNFL EKST ERET RQEY ALAM IQCK VLKQ LENL EQQK YDDE DISE DIKF LLEK LGES VQDL SSFD EYSS ELKS GRLE WSPV HKSE KFWR ENAV RLNE KNYE LLKI LTKL LEVS DDPQ VLAV AAHD VGEY VRHY PRGK RVIE QLGG KQLV MNHM HHED QQVR YNAL LAVQ KLMV HNWE YLGK QLQS EQPQ TAAA RS
Cellular Location:
Calculated MW:54kDa/55kDa
Observed MW:50kDa
Synonyms:ATP6V1H, CGI-11, MSTP042, NBP1, SFD, SFDalpha, SFDbeta, VMA13
Background:This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Western blot analysis of extracts of LO2 cells, using ATP6V1H antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Western blot analysis of extracts of LO2 cells, using ATP6V1H antibody at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
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